DOI: 10.1073/pnas.85.24.9381. The alpha and beta subunits of phosphorylase kinase are homologous: cDNA cloning and primary structure of the beta subunit.

The alpha and beta subunits of phosphorylase kinase are homologous: cDNA cloning and primary structure of the beta subunit.

10.1073/pnas.85.24.9381

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We have cloned cDNA molecules encoding the beta subunit of phosphorylase kinase (ATP:phosphorylase-b phosphotransferase; EC 2.7.1.38) from rabbit fast-twitch skeletal muscle and have determined the complete primary structure of the polypeptide by a combination of peptide and DNA sequencing. In the mature beta subunit, the initial methionine is replaced by an acetyl group. The subunit is composed of 1092 amino acids and has a calculated molecular mass of 125,205 Da. Alignment of its sequence with the alpha subunit of phosphorylase kinase reveals extensive regions of homology, but each molecule also possesses unique sequences. Two of the three phosphorylation sites known for the beta subunit and all seven phosphorylation sites known for the alpha subunit are located in these unique domains.

Bibliography

Kilimann, M. W., Zander, N. F., Kuhn, C. C., Crabb, J. W., Meyer, H. E., & Heilmeyer, L. M. (1988). The alpha and beta subunits of phosphorylase kinase are homologous: cDNA cloning and primary structure of the beta subunit. Proceedings of the National Academy of Sciences, 85(24), 9381â9385.

Authors 6

M W Kilimann (first)
N F Zander (additional)
C C Kuhn (additional)
J W Crabb (additional)
H E Meyer (additional)
L M Heilmeyer (additional)

References 0 Referenced 75

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 6:47 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:52 p.m.)
Indexed	2 months, 3 weeks ago (June 10, 2025, 3:04 p.m.)
Issued	36 years, 9 months ago (Dec. 1, 1988)
Published	36 years, 9 months ago (Dec. 1, 1988)
Published Online	36 years, 9 months ago (Dec. 1, 1988)
Published Print	36 years, 9 months ago (Dec. 1, 1988)

Funders 0

None

BibTeX

@article{Kilimann_1988, title={The alpha and beta subunits of phosphorylase kinase are homologous: cDNA cloning and primary structure of the beta subunit.}, volume={85}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.85.24.9381}, DOI={10.1073/pnas.85.24.9381}, number={24}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Kilimann, M W and Zander, N F and Kuhn, C C and Crabb, J W and Meyer, H E and Heilmeyer, L M}, year={1988}, month=dec, pages={9381–9385} }

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