DOI: 10.1073/pnas.85.2.502. Injection of an antibody against a p21 c-Ha-ras protein inhibits cleavage in axolotl eggs.

Injection of an antibody against a p21 c-Ha-ras protein inhibits cleavage in axolotl eggs.

10.1073/pnas.85.2.502

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The presence of a ras protein was demonstrated in cleaving axolotl eggs by selective immunoprecipitation with a polyclonal antibody against a peptide encoded by the c-Ha-ras oncogene, cellular homolog of the v-Ha-ras oncogene of Harvey rat sarcoma virus. Injection of this antibody into axolotl oocytes subjected to progesterone treatment does not prevent meiotic maturation. Injection of the same antibody into a blastomere of axolotl eggs at the 2- or 4-cell stage causes cleavage arrest in the descendants of the injected blastomere. Cytological observations of the injected eggs show, in the arrested blastomeres, enlarged nuclei always surrounded by an intact nuclear envelope and containing uncondensed chromatin. The possible role of ras protein in meiosis and mitosis is discussed.

Bibliography

Baltus, E., Hanocq-Quertier, J., Hanocq, F., & Brachet, J. (1988). Injection of an antibody against a p21 c-Ha-ras protein inhibits cleavage in axolotl eggs. Proceedings of the National Academy of Sciences, 85(2), 502â506.

Authors 4

E Baltus (first)
J Hanocq-Quertier (additional)
F Hanocq (additional)
J Brachet (additional)

References 0 Referenced 9

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 6:44 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:56 p.m.)
Indexed	1 year, 10 months ago (Oct. 23, 2023, 9:07 a.m.)
Issued	37 years, 7 months ago (Jan. 1, 1988)
Published	37 years, 7 months ago (Jan. 1, 1988)
Published Online	37 years, 7 months ago (Jan. 1, 1988)
Published Print	37 years, 7 months ago (Jan. 1, 1988)

Funders 0

None

BibTeX

@article{Baltus_1988, title={Injection of an antibody against a p21 c-Ha-ras protein inhibits cleavage in axolotl eggs.}, volume={85}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.85.2.502}, DOI={10.1073/pnas.85.2.502}, number={2}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Baltus, E and Hanocq-Quertier, J and Hanocq, F and Brachet, J}, year={1988}, month=jan, pages={502–506} }

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