DOI: 10.1073/pnas.85.18.6792. Cell adhesion induces expression of growth-associated genes in suspension-arrested fibroblasts.

Cell adhesion induces expression of growth-associated genes in suspension-arrested fibroblasts.

10.1073/pnas.85.18.6792

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A methylcellulose suspension system that prevents cell-surface contact with the substrate was used to study the role of cell adhesion in the regulation of proliferation. The nonadhesive conditions established by suspension culture cause BALB/c 3T3 (A31) cells to enter a G0 state of growth arrest within 48 hr as defined by an inhibition of DNA synthesis and a suppression of c-myc and histone mRNA expression. The adhesion of these suspension-arrested cells rapidly induces c-fos, c-myc, and actin gene expression. This stimulation did not depend on the presence of serum since the adhesion of suspension-arrested cells, in the absence of serum, also induced the expression of c-fos and c-myc mRNAs. In addition, adhesion onto fibronectin increased the number of cells able to respond to epidermal growth factor and insulin and progress into S phase. These results indicate that adhesion of suspension-arrested cells activates the G0/G1 transition independent of growth factors.

Bibliography

Dike, L. E., & Farmer, S. R. (1988). Cell adhesion induces expression of growth-associated genes in suspension-arrested fibroblasts. Proceedings of the National Academy of Sciences, 85(18), 6792â6796.

Authors 2

L E Dike (first)
S R Farmer (additional)

References 0 Referenced 124

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 6:41 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:41 p.m.)
Indexed	2 months, 1 week ago (June 26, 2025, 8:47 a.m.)
Issued	37 years ago (Sept. 1, 1988)
Published	37 years ago (Sept. 1, 1988)
Published Online	37 years ago (Sept. 1, 1988)
Published Print	37 years ago (Sept. 1, 1988)

Funders 0

None

BibTeX

@article{Dike_1988, title={Cell adhesion induces expression of growth-associated genes in suspension-arrested fibroblasts.}, volume={85}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.85.18.6792}, DOI={10.1073/pnas.85.18.6792}, number={18}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Dike, L E and Farmer, S R}, year={1988}, month=sep, pages={6792–6796} }

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