DOI: 10.1073/pnas.85.15.5610. Continuous, on-line DNA sequencing using oligodeoxynucleotide primers with multiple fluorophores.

Continuous, on-line DNA sequencing using oligodeoxynucleotide primers with multiple fluorophores.

10.1073/pnas.85.15.5610

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A method for sequencing DNA by using a difluoresceinated primer and laser excitation is described. Dideoxy protocols have been determined that provide sequences for 600 bases starting with base 1 with less than 1% error in a single load. Electrophoresis is at 20 W and the bands are detected 24 cm from the bottom of the loading well with a scanning fluorescence detector. Bands are imaged on a TV screen in two dimensions. The sequences can be read from the TV screen manually or semiautomatically by using a simple software program. The system allows more bases to be read with a lower error rate than any other reported automated sequencing method.

Bibliography

Brumbaugh, J. A., Middendorf, L. R., Grone, D. L., & Ruth, J. L. (1988). Continuous, on-line DNA sequencing using oligodeoxynucleotide primers with multiple fluorophores. Proceedings of the National Academy of Sciences, 85(15), 5610â5614.

Authors 4

J A Brumbaugh (first)
L R Middendorf (additional)
D L Grone (additional)
J L Ruth (additional)

References 0 Referenced 82

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 6:40 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:40 p.m.)
Indexed	1 year, 7 months ago (Jan. 13, 2024, 11:03 a.m.)
Issued	37 years ago (Aug. 1, 1988)
Published	37 years ago (Aug. 1, 1988)
Published Online	37 years ago (Aug. 1, 1988)
Published Print	37 years ago (Aug. 1, 1988)

Funders 0

None

BibTeX

@article{Brumbaugh_1988, title={Continuous, on-line DNA sequencing using oligodeoxynucleotide primers with multiple fluorophores.}, volume={85}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.85.15.5610}, DOI={10.1073/pnas.85.15.5610}, number={15}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Brumbaugh, J A and Middendorf, L R and Grone, D L and Ruth, J L}, year={1988}, month=aug, pages={5610–5614} }

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