DOI: 10.1073/pnas.84.23.8439. Stable and heritable inhibition of the expression of nopaline synthase in tobacco expressing antisense RNA

Stable and heritable inhibition of the expression of nopaline synthase in tobacco expressing antisense RNA

10.1073/pnas.84.23.8439

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Antisense nopaline synthase (nos) (D-nopaline synthase; EC 1.5.1.19) RNA is stably expressed from the cauliflower mosaic virus 35S promoter in transformed tobacco plants. The expression of a previously introduced wild-type nos gene is inhibited by the antisense RNA, with less nos enzyme activity detected (by a factor of 8-50) depending on the tissue analyzed. The steady-state levels of nos mRNA are reduced in the presence of the antisense RNA, implying that mRNA degradation is probably the main mode of action for the decrease in expression in this system. The antisense RNA-expressing gene and its inhibition of nos expression are shown to be heritable, demonstrating that it is a potentially useful method for the modification of phenotype.

Bibliography

Rothstein, S. J., DiMaio, J., Strand, M., & Rice, D. (1987). Stable and heritable inhibition of the expression of nopaline synthase in tobacco expressing antisense RNA. Proceedings of the National Academy of Sciences, 84(23), 8439â8443.

Authors 4

Steven J. Rothstein (first)
Joseph DiMaio (additional)
Micheline Strand (additional)
Douglas Rice (additional)

References 0 Referenced 69

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 6:29 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:12 p.m.)
Indexed	2 days, 18 hours ago (Aug. 31, 2025, 6:19 a.m.)
Issued	37 years, 9 months ago (Dec. 1, 1987)
Published	37 years, 9 months ago (Dec. 1, 1987)
Published Online	37 years, 9 months ago (Dec. 1, 1987)
Published Print	37 years, 9 months ago (Dec. 1, 1987)

Funders 0

None

BibTeX

@article{Rothstein_1987, title={Stable and heritable inhibition of the expression of nopaline synthase in tobacco expressing antisense RNA}, volume={84}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.84.23.8439}, DOI={10.1073/pnas.84.23.8439}, number={23}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Rothstein, Steven J. and DiMaio, Joseph and Strand, Micheline and Rice, Douglas}, year={1987}, month=dec, pages={8439–8443} }

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