DOI: 10.1073/pnas.84.14.4841. Metal-dependent folding of a single zinc finger from transcription factor IIIA.

Metal-dependent folding of a single zinc finger from transcription factor IIIA.

10.1073/pnas.84.14.4841

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A 30-amino acid peptide, which corresponds to the second "zinc finger" domain of transcription factor IIIA, has been synthesized and purified. This peptide folds in the presence of zinc: adding Zn2+ significantly changes the circular dichroism spectrum, and Zn2+ protects the peptide from tryptic digestion. The peptide also binds Co2+, and the absorption spectrum of the Co2+ complex suggests that a tetrahedral binding site is formed by two cysteines and two histidines. Experiments at higher temperatures (60-75 degrees C) suggest that these folded metal-peptide complexes are quite thermostable. The peptide shows some sequence-specific effects in DNase and methylation protection experiments. However, it does not give a clear "footprint," and some effects are observed in the absence of added zinc.

Bibliography

Frankel, A. D., Berg, J. M., & Pabo, C. O. (1987). Metal-dependent folding of a single zinc finger from transcription factor IIIA. Proceedings of the National Academy of Sciences, 84(14), 4841â4845.

Authors 3

A D Frankel (first)
J M Berg (additional)
C O Pabo (additional)

References 0 Referenced 216

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 6:22 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:31 p.m.)
Indexed	1 month, 2 weeks ago (July 8, 2025, 10:07 a.m.)
Issued	38 years, 1 month ago (July 1, 1987)
Published	38 years, 1 month ago (July 1, 1987)
Published Online	38 years, 1 month ago (July 1, 1987)
Published Print	38 years, 1 month ago (July 1, 1987)

Funders 0

None

BibTeX

@article{Frankel_1987, title={Metal-dependent folding of a single zinc finger from transcription factor IIIA.}, volume={84}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.84.14.4841}, DOI={10.1073/pnas.84.14.4841}, number={14}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Frankel, A D and Berg, J M and Pabo, C O}, year={1987}, month=jul, pages={4841–4845} }

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