DOI: 10.1073/pnas.84.14.4831. A human beta-actin expression vector system directs high-level accumulation of antisense transcripts.

A human beta-actin expression vector system directs high-level accumulation of antisense transcripts.

10.1073/pnas.84.14.4831

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We have constructed a mammalian expression vector consisting of 3 kilobases of the human beta-actin gene 5' flanking sequence plus 5' untranslated region and intervening sequence 1 linked at the 3' splice site to a short DNA polylinker sequence containing unique Sal I, HindIII, and BamHI restriction endonuclease sites followed by a simian virus 40 (SV40) polyadenylylation signal. Two derivatives, containing the selection markers obtained from pSV2gpt or pSV2neo, were also generated. We find that the promoter activity of this vector is a great or greater than that of the SV40 early promoter in a variety of human and rodent cells. The vector was used to generate gamma-actin and beta-tubulin antisense transcripts in human fibroblast cell lines. The antisense transcripts accumulate to levels comparable with that of the highly abundant gamma-actin and beta-tubulin mRNAs.

Bibliography

Gunning, P., Leavitt, J., Muscat, G., Ng, S. Y., & Kedes, L. (1987). A human beta-actin expression vector system directs high-level accumulation of antisense transcripts. Proceedings of the National Academy of Sciences, 84(14), 4831â4835.

Authors 5

P Gunning (first)
J Leavitt (additional)
G Muscat (additional)
S Y Ng (additional)
L Kedes (additional)

References 0 Referenced 463

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 6:22 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:31 p.m.)
Indexed	3 weeks, 6 days ago (Aug. 2, 2025, 12:10 a.m.)
Issued	38 years, 1 month ago (July 1, 1987)
Published	38 years, 1 month ago (July 1, 1987)
Published Online	38 years, 1 month ago (July 1, 1987)
Published Print	38 years, 1 month ago (July 1, 1987)

Funders 0

None

BibTeX

@article{Gunning_1987, title={A human beta-actin expression vector system directs high-level accumulation of antisense transcripts.}, volume={84}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.84.14.4831}, DOI={10.1073/pnas.84.14.4831}, number={14}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Gunning, P and Leavitt, J and Muscat, G and Ng, S Y and Kedes, L}, year={1987}, month=jul, pages={4831–4835} }

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