DOI: 10.1073/pnas.84.14.4767. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.

DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.

10.1073/pnas.84.14.4767

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A chemically modified phage T7 DNA polymerase has three properties that make it ideal for DNA sequencing by the chain-termination method. The enzyme is highly processive, catalyzing the polymerization of thousands of nucleotides without dissociating. By virtue of the modification the 3' to 5' exonuclease activity is eliminated. The modified polymerase efficiently uses nucleotide analogs that increase the electrophoretic resolution of bands in gels. Consequently, dideoxynucleotide-terminated fragments have highly uniform radioactive intensity throughout the range of a few to thousands of nucleotides in length. There is virtually no background due to terminations at pause sites or secondary-structure impediments. Processive synthesis with dITP in place of dGTP eliminates band compressions, making possible the unambiguous determination of sequences from a single orientation.

Bibliography

Tabor, S., & Richardson, C. C. (1987). DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proceedings of the National Academy of Sciences, 84(14), 4767â4771.

Authors 2

S Tabor (first)
C C Richardson (additional)

References 0 Referenced 1,245

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 6:22 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:31 p.m.)
Indexed	3 weeks, 3 days ago (Aug. 6, 2025, 8:15 a.m.)
Issued	38 years, 1 month ago (July 1, 1987)
Published	38 years, 1 month ago (July 1, 1987)
Published Online	38 years, 1 month ago (July 1, 1987)
Published Print	38 years, 1 month ago (July 1, 1987)

Funders 0

None

BibTeX

@article{Tabor_1987, title={DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.}, volume={84}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.84.14.4767}, DOI={10.1073/pnas.84.14.4767}, number={14}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Tabor, S and Richardson, C C}, year={1987}, month=jul, pages={4767–4771} }

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