DOI: 10.1073/pnas.84.1.131. Expression and assembly of functional bacterial luciferase in plants

Expression and assembly of functional bacterial luciferase in plants

10.1073/pnas.84.1.131

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The luxA and luxB structural genes of Vibrio harveyi luciferase [alkanal,reduced FMN:oxygen oxidoreductase (1-hydroxylating, luminescing), EC 1.14.14.3] were introduced into a plant expression vector and transferred into tobacco and carrot cells by Agrobacterium -mediated or direct DNA transformation. Simultaneous expression of the luxA and luxB genes was monitored by protein immunoblot analysis. Luciferase-mediated light emission provided evidence for the assembly of the two protein subunits into a functional dimeric enzyme in plant protoplasts, in transformed calli, and in leaves of transformed plants. Bacterial luciferase may provide a useful marker-gene system for the quantitative assay of coordinate gene expression in transgenic plants.

Bibliography

Koncz, C., Olsson, O., Langridge, W. H. R., Schell, J., & Szalay, A. A. (1987). Expression and assembly of functional bacterial luciferase in plants. Proceedings of the National Academy of Sciences, 84(1), 131â135.

Authors 5

Csaba Koncz (first)
Olof Olsson (additional)
William H. R. Langridge (additional)
Jeff Schell (additional)
Aladar A. Szalay (additional)

References 0 Referenced 99

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 6:17 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:24 p.m.)
Indexed	4 weeks ago (Aug. 5, 2025, 8:52 a.m.)
Issued	38 years, 8 months ago (Jan. 1, 1987)
Published	38 years, 8 months ago (Jan. 1, 1987)
Published Online	38 years, 8 months ago (Jan. 1, 1987)
Published Print	38 years, 8 months ago (Jan. 1, 1987)

Funders 0

None

BibTeX

@article{Koncz_1987, title={Expression and assembly of functional bacterial luciferase in plants}, volume={84}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.84.1.131}, DOI={10.1073/pnas.84.1.131}, number={1}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Koncz, Csaba and Olsson, Olof and Langridge, William H. R. and Schell, Jeff and Szalay, Aladar A.}, year={1987}, month=jan, pages={131–135} }

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