DOI: 10.1073/pnas.83.8.2571. Analysis of Agrobacterium tumefaciens virulence mutants in leaf discs.

Analysis of Agrobacterium tumefaciens virulence mutants in leaf discs.

10.1073/pnas.83.8.2571

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The leaf disc transformation system provides a simple means to score expression of various T-DNA markers within days of infection by Agrobacterium tumefaciens as well as long-term selection for growth of transformed callus and shoots. In this report, we describe the application of this system to evaluation of marker transfer and integration in a comprehensive set of defined avirulent mutants of A. tumefaciens. We conclude that virC is not essential when the T-DNA is present on a binary vector. The most likely explanation for this is that the virC gene product is involved in generation of a T-DNA intermediate.

Bibliography

Horsch, R. B., Klee, H. J., Stachel, S., Winans, S. C., Nester, E. W., Rogers, S. G., & Fraley, R. T. (1986). Analysis of Agrobacterium tumefaciens virulence mutants in leaf discs. Proceedings of the National Academy of Sciences, 83(8), 2571â2575.

Authors 7

R B Horsch (first)
H J Klee (additional)
S Stachel (additional)
S C Winans (additional)
E W Nester (additional)
S G Rogers (additional)
R T Fraley (additional)

References 0 Referenced 112

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 6:17 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:11 p.m.)
Indexed	1 month ago (July 24, 2025, 8:13 a.m.)
Issued	39 years, 4 months ago (April 1, 1986)
Published	39 years, 4 months ago (April 1, 1986)
Published Online	39 years, 4 months ago (April 1, 1986)
Published Print	39 years, 4 months ago (April 1, 1986)

Funders 0

None

BibTeX

@article{Horsch_1986, title={Analysis of Agrobacterium tumefaciens virulence mutants in leaf discs.}, volume={83}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.83.8.2571}, DOI={10.1073/pnas.83.8.2571}, number={8}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Horsch, R B and Klee, H J and Stachel, S and Winans, S C and Nester, E W and Rogers, S G and Fraley, R T}, year={1986}, month=apr, pages={2571–2575} }

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