DOI: 10.1073/pnas.83.5.1476. Microinjection of partially purified protein factor restores DNA damage specifically in group A of xeroderma pigmentosum cells.

Microinjection of partially purified protein factor restores DNA damage specifically in group A of xeroderma pigmentosum cells.

10.1073/pnas.83.5.1476

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Microinjection of cell extracts prepared from both human placenta and HeLa cells into xeroderma pigmentosum (XP) cells of complementation group A restores unscheduled DNA synthesis (UDS) in these cells after UV irradiation [de Jonge, A., Vermeulen, W., Klein, B. & Hoeijmakers, J. (1983) EMBO J. 2, 637-641]. These cells also showed normal resistance to UV irradiation. The half-life of the factors in the cell extracts corresponding to the UDS activity (factor A) was 14 hr in XP cells of group A, and the maximal level of UDS was exerted 2 hr after microinjection. The factors were sensitive to protease treatment but not to RNase treatment and were found to be approximately equal to 160 and approximately equal to 90 kDa by gel filtration. These two fractions of the factor(s) acted specifically in XP cells of complementation group A among complementation groups A, B, C, D, F, G, and probably E and H.

Bibliography

Yamaizumi, M., Sugano, T., Asahina, H., Okada, Y., & Uchida, T. (1986). Microinjection of partially purified protein factor restores DNA damage specifically in group A of xeroderma pigmentosum cells. Proceedings of the National Academy of Sciences, 83(5), 1476â1479.

Authors 5

M Yamaizumi (first)
T Sugano (additional)
H Asahina (additional)
Y Okada (additional)
T Uchida (additional)

References 0 Referenced 13

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 6:13 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:21 p.m.)
Indexed	1 year, 7 months ago (Jan. 13, 2024, 11:04 a.m.)
Issued	39 years, 6 months ago (March 1, 1986)
Published	39 years, 6 months ago (March 1, 1986)
Published Online	39 years, 6 months ago (March 1, 1986)
Published Print	39 years, 6 months ago (March 1, 1986)

Funders 0

None

BibTeX

@article{Yamaizumi_1986, title={Microinjection of partially purified protein factor restores DNA damage specifically in group A of xeroderma pigmentosum cells.}, volume={83}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.83.5.1476}, DOI={10.1073/pnas.83.5.1476}, number={5}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Yamaizumi, M and Sugano, T and Asahina, H and Okada, Y and Uchida, T}, year={1986}, month=mar, pages={1476–1479} }

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