DOI: 10.1073/pnas.83.2.251. Total DNA transcription in vitro: a procedure to detect highly repetitive and transcribable sequences with tRNA-like structures.

Total DNA transcription in vitro: a procedure to detect highly repetitive and transcribable sequences with tRNA-like structures.

10.1073/pnas.83.2.251

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Total DNAs from various animals were transcribed in vitro in a HeLa cell extract, and it was found that one to several discrete RNAs were transcribed by RNA polymerase III. With tortoise (Geoclemys reevessi) and newt (Cynops pyrrhogaster), distinct 6.5S and 8S RNAs were transcribed from these respective DNAs. Representative phage clones carrying the 6.5S and 8S RNA genes were isolated from genomic libraries of these animals, and the sequences of these genes were determined. The 5' parts of highly repetitive and transcribable sequences of tortoise and newt were found to have close resemblance to tRNALys1 (rabbit) gene (78% homology) and a tRNAGlu (Drosophila) gene (74% homology, not counting the aminoacyl stem region), respectively. The homologies extended to secondary structures, homologous nucleotides being located on similar secondary structures. It is proposed that many, if not all, highly repetitive and transcribable sequences detected by total DNA transcription have specific tRNA genes as their progenitors.

Bibliography

Endoh, H., & Okada, N. (1986). Total DNA transcription in vitro: a procedure to detect highly repetitive and transcribable sequences with tRNA-like structures. Proceedings of the National Academy of Sciences, 83(2), 251â255.

Authors 2

H Endoh (first)
N Okada (additional)

References 0 Referenced 45

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 6:07 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:33 p.m.)
Indexed	1 year, 1 month ago (Aug. 3, 2024, 8:46 a.m.)
Issued	39 years, 8 months ago (Jan. 1, 1986)
Published	39 years, 8 months ago (Jan. 1, 1986)
Published Online	39 years, 8 months ago (Jan. 1, 1986)
Published Print	39 years, 8 months ago (Jan. 1, 1986)

Funders 0

None

BibTeX

@article{Endoh_1986, title={Total DNA transcription in vitro: a procedure to detect highly repetitive and transcribable sequences with tRNA-like structures.}, volume={83}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.83.2.251}, DOI={10.1073/pnas.83.2.251}, number={2}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Endoh, H and Okada, N}, year={1986}, month=jan, pages={251–255} }

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