DOI: 10.1073/pnas.83.17.6233. Protein sequencing by tandem mass spectrometry.

Protein sequencing by tandem mass spectrometry.

10.1073/pnas.83.17.6233

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Methodology for determining amino acid sequences of proteins by tandem mass spectrometry is described. The approach involves enzymatic and/or chemical degradation of the protein to a collection of peptides which are then fractionated by high-performance liquid chromatography. Each fraction, containing as many as 10-15 peptides, is then analyzed directly, without further purification, by a combination of liquid secondary-ion/collision-activated dissociation mass spectrometry on a multianalyzer instrument. Interpretation of collision-activated dissociation mass spectra is described, and results are presented from a study of soluble peptides produced by treatment of apolipoprotein B with cyanogen bromide and trypsin.

Bibliography

Hunt, D. F., Yates, J. R., Shabanowitz, J., Winston, S., & Hauer, C. R. (1986). Protein sequencing by tandem mass spectrometry. Proceedings of the National Academy of Sciences, 83(17), 6233â6237.

Authors 5

D F Hunt (first)
J R Yates (additional)
J Shabanowitz (additional)
S Winston (additional)
C R Hauer (additional)

References 0 Referenced 934

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 6:05 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:11 p.m.)
Indexed	1 week, 6 days ago (Aug. 7, 2025, 4:42 p.m.)
Issued	38 years, 11 months ago (Sept. 1, 1986)
Published	38 years, 11 months ago (Sept. 1, 1986)
Published Online	38 years, 11 months ago (Sept. 1, 1986)
Published Print	38 years, 11 months ago (Sept. 1, 1986)

Funders 0

None

BibTeX

@article{Hunt_1986, title={Protein sequencing by tandem mass spectrometry.}, volume={83}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.83.17.6233}, DOI={10.1073/pnas.83.17.6233}, number={17}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Hunt, D F and Yates, J R and Shabanowitz, J and Winston, S and Hauer, C R}, year={1986}, month=sep, pages={6233–6237} }

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