DOI: 10.1073/pnas.82.8.2267. Purification and characterization of two cartilage-inducing factors from bovine demineralized bone.

Purification and characterization of two cartilage-inducing factors from bovine demineralized bone.

10.1073/pnas.82.8.2267

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Two naturally occurring peptides that induce chondrogenesis in culture have been purified to apparent homogeneity. These cartilage-inducing factors (CIF-A and CIF-B) were isolated from bovine demineralized bone by dissociative extraction, gel filtration, cation-exchange chromatography, and reversed-phase HPLC. CIF-A and CIF-B at concentrations of 1-10 ng/ml each induce embryonic rat mesenchymal cells in culture to assume a cartilage morphology and synthesize cartilage-specific proteoglycan and type II collagen. The amino acid compositions of CIF-A and CIF-B are similar but not identical. Both factors have an apparent Mr of 26,000, as determined by NaDodSO4/PAGE. In the presence of 2-mercaptoethanol, both are converted to species of about one-half that Mr, indicating that they are dimers of identical or very similar chains.

Bibliography

Seyedin, S. M., Thomas, T. C., Thompson, A. Y., Rosen, D. M., & Piez, K. A. (1985). Purification and characterization of two cartilage-inducing factors from bovine demineralized bone. Proceedings of the National Academy of Sciences, 82(8), 2267â2271.

Authors 5

S M Seyedin (first)
T C Thomas (additional)
A Y Thompson (additional)
D M Rosen (additional)
K A Piez (additional)

References 0 Referenced 343

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 5:55 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:11 p.m.)
Indexed	3 months, 2 weeks ago (May 19, 2025, 7:45 a.m.)
Issued	40 years, 5 months ago (April 1, 1985)
Published	40 years, 5 months ago (April 1, 1985)
Published Online	40 years, 5 months ago (April 1, 1985)
Published Print	40 years, 5 months ago (April 1, 1985)

Funders 0

None

BibTeX

@article{Seyedin_1985, title={Purification and characterization of two cartilage-inducing factors from bovine demineralized bone.}, volume={82}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.82.8.2267}, DOI={10.1073/pnas.82.8.2267}, number={8}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Seyedin, S M and Thomas, T C and Thompson, A Y and Rosen, D M and Piez, K A}, year={1985}, month=apr, pages={2267–2271} }

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