DOI: 10.1073/pnas.82.6.1585. Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.

Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.

10.1073/pnas.82.6.1585

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

An oligonucleotide hybridization procedure has been developed that eliminates the preferential melting of A X T versus G X C base pairs, allowing the stringency of the hybridization to be controlled as a function of probe length only. This technique, which uses tetramethylammonium chloride, is especially helpful whenever a highly complex library is screened with a pool of oligonucleotide probes, which usually vary widely in base composition. The procedure can also be applied advantageously whenever an exact match to an oligonucleotide probe is desired, such as in screening for clones having as little as a single-base alteration generated by in vitro mutagenesis.

Bibliography

Wood, W. I., Gitschier, J., Lasky, L. A., & Lawn, R. M. (1985). Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries. Proceedings of the National Academy of Sciences, 82(6), 1585â1588.

Authors 4

W I Wood (first)
J Gitschier (additional)
L A Lasky (additional)
R M Lawn (additional)

References 0 Referenced 436

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 3:45 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:20 p.m.)
Indexed	5 months ago (March 24, 2025, 2:49 a.m.)
Issued	40 years, 5 months ago (March 1, 1985)
Published	40 years, 5 months ago (March 1, 1985)
Published Online	40 years, 5 months ago (March 1, 1985)
Published Print	40 years, 5 months ago (March 1, 1985)

Funders 0

None

BibTeX

@article{Wood_1985, title={Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.}, volume={82}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.82.6.1585}, DOI={10.1073/pnas.82.6.1585}, number={6}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Wood, W I and Gitschier, J and Lasky, L A and Lawn, R M}, year={1985}, month=mar, pages={1585–1588} }

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