DOI: 10.1073/pnas.82.4.1069. Feedback regulation of rRNA and tRNA synthesis and accumulation of free ribosomes after conditional expression of rRNA genes.

Feedback regulation of rRNA and tRNA synthesis and accumulation of free ribosomes after conditional expression of rRNA genes.

10.1073/pnas.82.4.1069

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We have constructed a conditional rRNA gene expression system by fusing a plasmid-encoded rrnB operon to the lambda PL promoter/operator. It was thereby possible to study the events that lead to the regulation of chromosomal rRNA and tRNA synthesis after overproduction of rRNA. rRNA induction resulted in a 2-fold increase in 30S and 50S free ribosomal subunits, whereas the polysome fraction was unaffected. Overproduction of rRNA and "free" ribosomes produced a large repression of rRNA and tRNA synthesis from chromosomal genes and a smaller increase in the concentration of guanosine tetraphosphate. These results lend support to the ribosome feedback regulation model: rRNA and tRNA operons are negatively regulated, either directly or through some intermediate, by free, nontranslating ribosomes.

Bibliography

Gourse, R. L., Takebe, Y., Sharrock, R. A., & Nomura, M. (1985). Feedback regulation of rRNA and tRNA synthesis and accumulation of free ribosomes after conditional expression of rRNA genes. Proceedings of the National Academy of Sciences, 82(4), 1069â1073.

Authors 4

R L Gourse (first)
Y Takebe (additional)
R A Sharrock (additional)
M Nomura (additional)

References 0 Referenced 102

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 5:52 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:25 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:46 p.m.)
Issued	40 years, 6 months ago (Feb. 1, 1985)
Published	40 years, 6 months ago (Feb. 1, 1985)
Published Online	40 years, 6 months ago (Feb. 1, 1985)
Published Print	40 years, 6 months ago (Feb. 1, 1985)

Funders 0

None

BibTeX

@article{Gourse_1985, title={Feedback regulation of rRNA and tRNA synthesis and accumulation of free ribosomes after conditional expression of rRNA genes.}, volume={82}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.82.4.1069}, DOI={10.1073/pnas.82.4.1069}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Gourse, R L and Takebe, Y and Sharrock, R A and Nomura, M}, year={1985}, month=feb, pages={1069–1073} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        2
      ]
    ],
    "date-time": "2025-07-02T18:46:21Z",
    "timestamp": 1751481981734
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "4",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1985,
        2
      ]
    ]
  },
  "abstract": "<jats:p>We have constructed a conditional rRNA gene expression system by fusing a plasmid-encoded rrnB operon to the lambda PL promoter/operator. It was thereby possible to study the events that lead to the regulation of chromosomal rRNA and tRNA synthesis after overproduction of rRNA. rRNA induction resulted in a 2-fold increase in 30S and 50S free ribosomal subunits, whereas the polysome fraction was unaffected. Overproduction of rRNA and \"free\" ribosomes produced a large repression of rRNA and tRNA synthesis from chromosomal genes and a smaller increase in the concentration of guanosine tetraphosphate. These results lend support to the ribosome feedback regulation model: rRNA and tRNA operons are negatively regulated, either directly or through some intermediate, by free, nontranslating ribosomes.</jats:p>",
  "DOI": "10.1073/pnas.82.4.1069",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T09:52:20Z",
    "timestamp": 1149069140000
  },
  "page": "1069-1073",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 102,
  "title": "Feedback regulation of rRNA and tRNA synthesis and accumulation of free ribosomes after conditional expression of rRNA genes.",
  "prefix": "10.1073",
  "volume": "82",
  "author": [
    {
      "given": "R L",
      "family": "Gourse",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Y",
      "family": "Takebe",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "R A",
      "family": "Sharrock",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "M",
      "family": "Nomura",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1985,
        2
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.82.4.1069",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T16:25:58Z",
    "timestamp": 1649867158000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.82.4.1069"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1985,
        2
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "4",
    "published-print": {
      "date-parts": [
        [
          1985,
          2
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.82.4.1069"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.82.4.1069",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1985,
        2
      ]
    ]
  },
  "assertion": [
    {
      "value": "1985-02-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}