DOI: 10.1073/pnas.82.3.737. A synthetic peptide substrate specific for casein kinase II.

A synthetic peptide substrate specific for casein kinase II.

10.1073/pnas.82.3.737

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A synthetic peptide having the sequence Arg-Arg-Arg-Glu-Glu-Thr-Glu-Glu-Glu was found to serve as a convenient substrate for the protein kinase generally referred to as casein kinase II. The enzyme exhibited an apparent Km of 500 microM for the peptide, as compared to an apparent Km of 50 microM for casein. The maximum velocities for phosphorylation of the peptide and of casein were similar. The peptide was not phosphorylated by any of eight other protein kinases, all of which were shown to be active toward their known substrates. The peptide was used to monitor activity during steps in the purification of casein kinase II from bovine liver. These experiments demonstrated that with this peptide it is now possible to obtain specific measurements of casein kinase II activity in crude enzyme preparations.

Bibliography

Kuenzel, E. A., & Krebs, E. G. (1985). A synthetic peptide substrate specific for casein kinase II. Proceedings of the National Academy of Sciences, 82(3), 737â741.

Authors 2

E A Kuenzel (first)
E G Krebs (additional)

References 0 Referenced 153

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 5:51 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:30 p.m.)
Indexed	3 months, 2 weeks ago (May 20, 2025, 10:30 a.m.)
Issued	40 years, 7 months ago (Feb. 1, 1985)
Published	40 years, 7 months ago (Feb. 1, 1985)
Published Online	40 years, 7 months ago (Feb. 1, 1985)
Published Print	40 years, 7 months ago (Feb. 1, 1985)

Funders 0

None

BibTeX

@article{Kuenzel_1985, title={A synthetic peptide substrate specific for casein kinase II.}, volume={82}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.82.3.737}, DOI={10.1073/pnas.82.3.737}, number={3}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Kuenzel, E A and Krebs, E G}, year={1985}, month=feb, pages={737–741} }

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