DOI: 10.1073/pnas.82.24.8330. Evidence for functional interaction between domains II and V of 23S ribosomal RNA from an erythromycin-resistant mutant.

Evidence for functional interaction between domains II and V of 23S ribosomal RNA from an erythromycin-resistant mutant.

10.1073/pnas.82.24.8330

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A mutation affording low levels of erythromycin resistance has been obtained by in vitro hydroxylamine mutagenesis of a cloned ribosomal RNA operon from Escherichia coli. The site of the mutational event responsible for antibiotic resistance was localized to the gene region encoding domain II of 23S rRNA by replacement of restriction fragments in the wild-type plasmid by corresponding fragments from the mutant plasmid. DNA sequencing showed that positions 1219-1230 of the 23S rRNA gene are deleted in the mutant. Since all previously characterized rRNA mutations conferring resistance to erythromycin show changes exclusively in domain V, our present findings provide direct evidence for functional interaction between domains II and V of 23S rRNA.

Bibliography

Douthwaite, S., Prince, J. B., & Noller, H. F. (1985). Evidence for functional interaction between domains II and V of 23S ribosomal RNA from an erythromycin-resistant mutant. Proceedings of the National Academy of Sciences, 82(24), 8330â8334.

Authors 3

S Douthwaite (first)
J B Prince (additional)
H F Noller (additional)

References 0 Referenced 35

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 5:50 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:23 p.m.)
Indexed	2 months ago (July 2, 2025, 2:46 p.m.)
Issued	39 years, 9 months ago (Dec. 1, 1985)
Published	39 years, 9 months ago (Dec. 1, 1985)
Published Online	39 years, 9 months ago (Dec. 1, 1985)
Published Print	39 years, 9 months ago (Dec. 1, 1985)

Funders 0

None

BibTeX

@article{Douthwaite_1985, title={Evidence for functional interaction between domains II and V of 23S ribosomal RNA from an erythromycin-resistant mutant.}, volume={82}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.82.24.8330}, DOI={10.1073/pnas.82.24.8330}, number={24}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Douthwaite, S and Prince, J B and Noller, H F}, year={1985}, month=dec, pages={8330–8334} }

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