DOI: 10.1073/pnas.82.22.7520. Expression at the cell surface of biologically active fusion and hemagglutinin/neuraminidase proteins of the paramyxovirus simian virus 5 from cloned cDNA.

Expression at the cell surface of biologically active fusion and hemagglutinin/neuraminidase proteins of the paramyxovirus simian virus 5 from cloned cDNA.

10.1073/pnas.82.22.7520

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

cDNAs encoding the mRNAs for the fusion protein (F) and the hemagglutinin/neuraminidase protein (HN) of the paramyxovirus simian virus 5 have been inserted into a eukaryotic expression vector under the control of the simian virus 40 late promoter. The F and HN proteins synthesized in recombinant infected cells are indistinguishable in terms of electrophoretic mobility and glycosylation from the proteins synthesized in simian virus 5-infected cells. In addition, the expressed F and HN proteins have been shown to be anchored in the plasma membrane in a biologically active form by indirect live cell immunofluorescence, the F-mediated formation of syncytia, and the ability of HN to cause the hemadsorption of erythrocytes to the infected cell surface.

Bibliography

Paterson, R. G., Hiebert, S. W., & Lamb, R. A. (1985). Expression at the cell surface of biologically active fusion and hemagglutinin/neuraminidase proteins of the paramyxovirus simian virus 5 from cloned cDNA. Proceedings of the National Academy of Sciences, 82(22), 7520â7524.

Authors 3

R G Paterson (first)
S W Hiebert (additional)
R A Lamb (additional)

References 0 Referenced 92

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 5:50 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:19 p.m.)
Indexed	1 year, 2 months ago (June 13, 2024, 3:18 p.m.)
Issued	39 years, 9 months ago (Nov. 1, 1985)
Published	39 years, 9 months ago (Nov. 1, 1985)
Published Online	39 years, 9 months ago (Nov. 1, 1985)
Published Print	39 years, 9 months ago (Nov. 1, 1985)

Funders 0

None

BibTeX

@article{Paterson_1985, title={Expression at the cell surface of biologically active fusion and hemagglutinin/neuraminidase proteins of the paramyxovirus simian virus 5 from cloned cDNA.}, volume={82}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.82.22.7520}, DOI={10.1073/pnas.82.22.7520}, number={22}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Paterson, R G and Hiebert, S W and Lamb, R A}, year={1985}, month=nov, pages={7520–7524} }

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