DOI: 10.1073/pnas.82.19.6507. Primary structure of bovine pituitary basic fibroblast growth factor (FGF) and comparison with the amino-terminal sequence of bovine brain acidic FGF.

Primary structure of bovine pituitary basic fibroblast growth factor (FGF) and comparison with the amino-terminal sequence of bovine brain acidic FGF.

10.1073/pnas.82.19.6507

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The two major mitogenic polypeptides for endothelial cells have been purified to homogeneity. The complete primary structure of bovine pituitary basic fibroblast growth factor (FGF) and the amino-terminal amino acid sequence of bovine brain acidic FGF have been established by gas-phase sequence analyses. Homogeneous preparations of these polypeptides are potent mitogens (basic FGF, ED50 approximately equal to 60 pg/ml; acidic FGF ED50 approximately equal to 6000 pg/ml) for many diverse cell types including capillary endothelial cells, vascular smooth muscle cells, and adrenocortical and granulosa cells; in vivo, basic FGF is a powerful angiogenic agent in the chick chorioallantoic membrane assay. The available protein sequence data demonstrate the existence of significant structural homology between the two polypeptides.

Bibliography

Esch, F., Baird, A., Ling, N., Ueno, N., Hill, F., Denoroy, L., Klepper, R., Gospodarowicz, D., BÃ¶hlen, P., & Guillemin, R. (1985). Primary structure of bovine pituitary basic fibroblast growth factor (FGF) and comparison with the amino-terminal sequence of bovine brain acidic FGF. Proceedings of the National Academy of Sciences, 82(19), 6507â6511.

Authors 10

F Esch (first)
A Baird (additional)
N Ling (additional)
N Ueno (additional)
F Hill (additional)
L Denoroy (additional)
R Klepper (additional)
D Gospodarowicz (additional)
P Böhlen (additional)
R Guillemin (additional)

References 0 Referenced 417

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 5:47 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:18 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 3:08 p.m.)
Issued	39 years, 10 months ago (Oct. 1, 1985)
Published	39 years, 10 months ago (Oct. 1, 1985)
Published Online	39 years, 10 months ago (Oct. 1, 1985)
Published Print	39 years, 10 months ago (Oct. 1, 1985)

Funders 0

None

BibTeX

@article{Esch_1985, title={Primary structure of bovine pituitary basic fibroblast growth factor (FGF) and comparison with the amino-terminal sequence of bovine brain acidic FGF.}, volume={82}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.82.19.6507}, DOI={10.1073/pnas.82.19.6507}, number={19}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Esch, F and Baird, A and Ling, N and Ueno, N and Hill, F and Denoroy, L and Klepper, R and Gospodarowicz, D and Böhlen, P and Guillemin, R}, year={1985}, month=oct, pages={6507–6511} }

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