DOI: 10.1073/pnas.82.18.6148. Efficient insertion of genes into the mouse germ line via retroviral vectors.

Efficient insertion of genes into the mouse germ line via retroviral vectors.

10.1073/pnas.82.18.6148

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We present a general strategy for the efficient insertion of recombinant retroviral vector DNA into the mouse germ line via infection of preimplantation mouse embryos. Transgenic mice were generated that harbor a replication-competent recombinant retrovirus (delta Mo + Py M-MuLV) that lacks the Moloney murine leukemia virus (M-MuLV)-type enhancer sequence in the long terminal repeat (LTR). Instead, the LTR contains an enhancer element that permits polyoma virus F101 to grow in undifferentiated F9 embryonal carcinoma cells. Expression studies in different tissues of animals transgenic for delta Mo + Py M-MuLV indicate possibilities to target and modulate expression of retroviral recombinants in mice via their LTR enhancer sequences. In addition, 16 transgenic mice were generated that harbor proviral DNA of a defective recombinant retrovirus carrying a mutant dihydrofolate reductase gene.

Bibliography

van der Putten, H., Botteri, F. M., Miller, A. D., Rosenfeld, M. G., Fan, H., Evans, R. M., & Verma, I. M. (1985). Efficient insertion of genes into the mouse germ line via retroviral vectors. Proceedings of the National Academy of Sciences, 82(18), 6148â6152.

Authors 7

H van der Putten (first)
F M Botteri (additional)
A D Miller (additional)
M G Rosenfeld (additional)
H Fan (additional)
R M Evans (additional)
I M Verma (additional)

References 0 Referenced 85

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 5:45 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:28 p.m.)
Indexed	4 months, 3 weeks ago (April 8, 2025, 9:58 p.m.)
Issued	40 years ago (Sept. 1, 1985)
Published	40 years ago (Sept. 1, 1985)
Published Online	40 years ago (Sept. 1, 1985)
Published Print	40 years ago (Sept. 1, 1985)

Funders 0

None

BibTeX

@article{van_der_Putten_1985, title={Efficient insertion of genes into the mouse germ line via retroviral vectors.}, volume={82}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.82.18.6148}, DOI={10.1073/pnas.82.18.6148}, number={18}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={van der Putten, H and Botteri, F M and Miller, A D and Rosenfeld, M G and Fan, H and Evans, R M and Verma, I M}, year={1985}, month=sep, pages={6148–6152} }

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