DOI: 10.1073/pnas.82.16.5295. In vitro mutagenesis of the v-sis transforming gene defines functional domains of its growth factor-related product.

In vitro mutagenesis of the v-sis transforming gene defines functional domains of its growth factor-related product.

10.1073/pnas.82.16.5295

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The polypeptide sequence of the v-sis transforming gene product of simian sarcoma virus (SSV) can be divided into four regions that are likely to represent structural domains of the protein. Mutations were generated in the SSV nucleotide sequence to assay the extent or function of each of these regions. The results indicate that the helper virus-derived amino-terminal sequence as well as a core region homologous to polypeptide chain 2 of platelet-derived growth factor (PDGF) are required for the transforming function of the protein. Products of transforming but not nontransforming mutants formed dimer structures conformationally analogous to biologically active PDGF.

Bibliography

King, C. R., Giese, N. A., Robbins, K. C., & Aaronson, S. A. (1985). In vitro mutagenesis of the v-sis transforming gene defines functional domains of its growth factor-related product. Proceedings of the National Academy of Sciences, 82(16), 5295â5299.

Authors 4

C R King (first)
N A Giese (additional)
K C Robbins (additional)
S A Aaronson (additional)

References 0 Referenced 30

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 5:44 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:01 p.m.)
Indexed	1 year, 1 month ago (July 10, 2024, 4:38 p.m.)
Issued	40 years, 1 month ago (Aug. 1, 1985)
Published	40 years, 1 month ago (Aug. 1, 1985)
Published Online	40 years, 1 month ago (Aug. 1, 1985)
Published Print	40 years, 1 month ago (Aug. 1, 1985)

Funders 0

None

BibTeX

@article{King_1985, title={In vitro mutagenesis of the v-sis transforming gene defines functional domains of its growth factor-related product.}, volume={82}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.82.16.5295}, DOI={10.1073/pnas.82.16.5295}, number={16}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={King, C R and Giese, N A and Robbins, K C and Aaronson, S A}, year={1985}, month=aug, pages={5295–5299} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        7,
        10
      ]
    ],
    "date-time": "2024-07-10T20:38:38Z",
    "timestamp": 1720643918891
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "16",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1985,
        8
      ]
    ]
  },
  "abstract": "<jats:p>The polypeptide sequence of the v-sis transforming gene product of simian sarcoma virus (SSV) can be divided into four regions that are likely to represent structural domains of the protein. Mutations were generated in the SSV nucleotide sequence to assay the extent or function of each of these regions. The results indicate that the helper virus-derived amino-terminal sequence as well as a core region homologous to polypeptide chain 2 of platelet-derived growth factor (PDGF) are required for the transforming function of the protein. Products of transforming but not nontransforming mutants formed dimer structures conformationally analogous to biologically active PDGF.</jats:p>",
  "DOI": "10.1073/pnas.82.16.5295",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T09:44:25Z",
    "timestamp": 1149068665000
  },
  "page": "5295-5299",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 30,
  "title": "In vitro mutagenesis of the v-sis transforming gene defines functional domains of its growth factor-related product.",
  "prefix": "10.1073",
  "volume": "82",
  "author": [
    {
      "given": "C R",
      "family": "King",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "N A",
      "family": "Giese",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "K C",
      "family": "Robbins",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "S A",
      "family": "Aaronson",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1985,
        8
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.82.16.5295",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T16:01:03Z",
    "timestamp": 1649865663000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.82.16.5295"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1985,
        8
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "16",
    "published-print": {
      "date-parts": [
        [
          1985,
          8
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.82.16.5295"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.82.16.5295",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1985,
        8
      ]
    ]
  },
  "assertion": [
    {
      "value": "1985-08-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}