DOI: 10.1073/pnas.82.15.4920. Characterization and sequence of the promoter region of the human epidermal growth factor receptor gene.

Characterization and sequence of the promoter region of the human epidermal growth factor receptor gene.

10.1073/pnas.82.15.4920

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The promoter region of the epidermal growth factor (EGF) receptor has been identified by in vitro transcription using EGF receptor genomic DNA fragments as template and by primer extension and nuclease S1 mapping using EGF receptor mRNA. Six transcriptional start sites were identified. DNA sequence analysis shows that the promoter region contains neither a "TATA box" nor a "CAAT box," has an extremely high G+C content (88%), and contains five CCGCCC repeats and four (TCC)TCCTCCTCC repeats. This promoter region is situated close to or within a DNase I-hypersensitive site in A431 human epidermoid carcinoma cells, which overproduce the EGF receptor. The EGF receptor gene promoter has some resemblance to the promoter of the hydroxymethylglutaryl-CoA reductase gene and the early promoter of simian virus 40. This similarity may offer a clue to the mechanism by which the receptor gene is regulated.

Bibliography

Ishii, S., Xu, Y. H., Stratton, R. H., Roe, B. A., Merlino, G. T., & Pastan, I. (1985). Characterization and sequence of the promoter region of the human epidermal growth factor receptor gene. Proceedings of the National Academy of Sciences, 82(15), 4920â4924.

Authors 6

S Ishii (first)
Y H Xu (additional)
R H Stratton (additional)
B A Roe (additional)
G T Merlino (additional)
I Pastan (additional)

References 0 Referenced 226

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 5:44 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:49 a.m.)
Indexed	1 month, 1 week ago (July 26, 2025, 5:20 a.m.)
Issued	40 years, 1 month ago (Aug. 1, 1985)
Published	40 years, 1 month ago (Aug. 1, 1985)
Published Online	40 years, 1 month ago (Aug. 1, 1985)
Published Print	40 years, 1 month ago (Aug. 1, 1985)

Funders 0

None

BibTeX

@article{Ishii_1985, title={Characterization and sequence of the promoter region of the human epidermal growth factor receptor gene.}, volume={82}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.82.15.4920}, DOI={10.1073/pnas.82.15.4920}, number={15}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Ishii, S and Xu, Y H and Stratton, R H and Roe, B A and Merlino, G T and Pastan, I}, year={1985}, month=aug, pages={4920–4924} }

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