DOI: 10.1073/pnas.82.13.4326. Expression of a functional influenza viral cap-recognizing protein by using a bovine papilloma virus vector.

Expression of a functional influenza viral cap-recognizing protein by using a bovine papilloma virus vector.

10.1073/pnas.82.13.4326

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The gene for the influenza viral PB2 protein, which recognizes and binds the 5'-terminal cap 1 structures (m7GpppNm) on eukaryotic mRNAs, was inserted into a bovine papilloma virus vector under the control of a mouse metallothionein I (MT-I) promoter. After transfection of this vector into mouse NIH 3T3 cells, a cell line, cPB2, was obtained that produces PB2-specific mRNA and authentic PB2 protein. Induction of the MT-I promoter with CdCl2 causes about a 10-fold increase in PB2 mRNA and protein levels. The expressed PB2 protein is functional, as it relieves the block in viral mRNA synthesis exhibited by a temperature-sensitive viral mutant containing a cap-binding defect in the PB2 protein. This demonstrates complementation of a function of a negative-strand RNA virus by a gene product expressed in a cell line from recombinant DNA.

Bibliography

Braam-Markson, J., Jaudon, C., & Krug, R. M. (1985). Expression of a functional influenza viral cap-recognizing protein by using a bovine papilloma virus vector. Proceedings of the National Academy of Sciences, 82(13), 4326â4330.

Authors 3

J Braam-Markson (first)
C Jaudon (additional)
R M Krug (additional)

References 0 Referenced 10

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 3:43 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:52 a.m.)
Indexed	1 year, 10 months ago (Oct. 16, 2023, 7:56 p.m.)
Issued	40 years, 2 months ago (July 1, 1985)
Published	40 years, 2 months ago (July 1, 1985)
Published Online	40 years, 2 months ago (July 1, 1985)
Published Print	40 years, 2 months ago (July 1, 1985)

Funders 0

None

BibTeX

@article{Braam_Markson_1985, title={Expression of a functional influenza viral cap-recognizing protein by using a bovine papilloma virus vector.}, volume={82}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.82.13.4326}, DOI={10.1073/pnas.82.13.4326}, number={13}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Braam-Markson, J and Jaudon, C and Krug, R M}, year={1985}, month=jul, pages={4326–4330} }

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