DOI: 10.1073/pnas.82.10.3192. Enzyme-catalyzed processes in organic solvents.

Enzyme-catalyzed processes in organic solvents.

10.1073/pnas.82.10.3192

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Three different lipases (porcine pancreatic, yeast, and mold) can vigorously act as catalysts in a number of nearly anhydrous organic solvents. Various transesterification reactions catalyzed by porcine pancreatic lipase in hexane obey Michaelis-Menten kinetics. The dependence of the catalytic activity of the enzyme in organic media on the pH of the aqueous solution from which it was recovered is bell-shaped, with the maximum coinciding with the pH optimum of the enzymatic activity in water. The catalytic power exhibited by the lipases in organic solvents is comparable to that displayed in water. In addition to transesterification, lipases can catalyze several other processes in organic media including esterification, aminolysis, acyl exchange, thiotransesterification, and oximolysis; some of these reactions proceed to an appreciable extent only in nonaqueous solvents.

Bibliography

Zaks, A., & Klibanov, A. M. (1985). Enzyme-catalyzed processes in organic solvents. Proceedings of the National Academy of Sciences, 82(10), 3192â3196.

Authors 2

A Zaks (first)
A M Klibanov (additional)

References 0 Referenced 1,052

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 5:40 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:52 a.m.)
Indexed	6 days, 12 hours ago (Aug. 23, 2025, 9:32 p.m.)
Issued	40 years, 3 months ago (May 1, 1985)
Published	40 years, 3 months ago (May 1, 1985)
Published Online	40 years, 3 months ago (May 1, 1985)
Published Print	40 years, 3 months ago (May 1, 1985)

Funders 0

None

BibTeX

@article{Zaks_1985, title={Enzyme-catalyzed processes in organic solvents.}, volume={82}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.82.10.3192}, DOI={10.1073/pnas.82.10.3192}, number={10}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Zaks, A and Klibanov, A M}, year={1985}, month=may, pages={3192–3196} }

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