DOI: 10.1073/pnas.81.9.2772. Dimeric structure of single chloride channels from Torpedo electroplax.

Dimeric structure of single chloride channels from Torpedo electroplax.

10.1073/pnas.81.9.2772

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The inhibition by 4,4'-diisothiocyano-2,2'-stilbenedisulfonate (DIDS) of Cl- channels from Torpedo electroplax incorporated in planar phospholipid bilayer membranes is studied. DIDS irreversibly and rapidly inhibits the macroscopic conductance of membranes containing many channels. At the single-channel level, the effect of DIDS is more complicated. The uninhibited single channel displays three "substates" of conductances 20, 10, and 0 pS. Short exposure (5-30 s) to 10 microM DIDS converts this three-level active channel into a "conventional" channel of 10-pS conductance. Longer exposure eliminates all channel fluctuations. The results are taken as strong evidence that the Cl- channel is constructed as a functional dimer of identical protein subunits.

Bibliography

Miller, C., & White, M. M. (1984). Dimeric structure of single chloride channels from Torpedo electroplax. Proceedings of the National Academy of Sciences, 81(9), 2772â2775.

Authors 2

C Miller (first)
M M White (additional)

References 0 Referenced 195

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 5:40 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:42 a.m.)
Indexed	1 month, 1 week ago (July 25, 2025, 6:50 a.m.)
Issued	41 years, 4 months ago (May 1, 1984)
Published	41 years, 4 months ago (May 1, 1984)
Published Online	41 years, 4 months ago (May 1, 1984)
Published Print	41 years, 4 months ago (May 1, 1984)

Funders 0

None

BibTeX

@article{Miller_1984, title={Dimeric structure of single chloride channels from Torpedo electroplax.}, volume={81}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.81.9.2772}, DOI={10.1073/pnas.81.9.2772}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Miller, C and White, M M}, year={1984}, month=may, pages={2772–2775} }

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