DOI: 10.1073/pnas.81.6.1715. pH-sensitive liposomes: acid-induced liposome fusion.

pH-sensitive liposomes: acid-induced liposome fusion.

10.1073/pnas.81.6.1715

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Sonicated unilamellar liposomes containing phosphatidylethanolamine and palmitoylhomocysteine fuse rapidly when the medium pH is lowered from 7 to 5. Liposome fusion was demonstrated by (i) mixing of the liposomal lipids as shown by resonance energy transfer, (ii) gel filtration, and (iii) electron microscopy. The pH-sensitive fusion of liposomes was observed only when palmitoylhomocysteine (greater than or equal to 20 mol%) was present in the liposomes. The presence of phosphatidyl-ethanolamine in the liposomes greatly enhanced fusion whereas the presence of phosphatidylcholine inhibited fusion. During fusion of liposomes containing phosphatidylethanolamine and palmitoylhomocysteine (8:2, mol/mol), almost all of the encapsulated calcein was released. Inclusion of cholesterol (40 mol%) in the liposomes substantially decreased leakage without impairing fusion.

Bibliography

Connor, J., Yatvin, M. B., & Huang, L. (1984). pH-sensitive liposomes: acid-induced liposome fusion. Proceedings of the National Academy of Sciences, 81(6), 1715â1718.

Authors 3

J Connor (first)
M B Yatvin (additional)
L Huang (additional)

References 0 Referenced 209

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 5:38 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:55 a.m.)
Indexed	1 month ago (Aug. 2, 2025, 1:11 a.m.)
Issued	41 years, 6 months ago (March 1, 1984)
Published	41 years, 6 months ago (March 1, 1984)
Published Online	41 years, 6 months ago (March 1, 1984)
Published Print	41 years, 6 months ago (March 1, 1984)

Funders 0

None

BibTeX

@article{Connor_1984, title={pH-sensitive liposomes: acid-induced liposome fusion.}, volume={81}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.81.6.1715}, DOI={10.1073/pnas.81.6.1715}, number={6}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Connor, J and Yatvin, M B and Huang, L}, year={1984}, month=mar, pages={1715–1718} }

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