DOI: 10.1073/pnas.81.24.7907. Nucleotide sequence of human monocyte interleukin 1 precursor cDNA.

Nucleotide sequence of human monocyte interleukin 1 precursor cDNA.

10.1073/pnas.81.24.7907

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Interleukin 1 (IL-1) is a protein with several biological activities regulating host defense and immune responses. We report here the isolation of human IL-1 cDNA. It encodes a precursor polypeptide of 269 amino acids (30,747 Mr). mRNA isolated by hybridization to this cDNA was translated in a reticulocyte cell-free system, yielding immunoprecipitable IL-1. Furthermore, this hybrid-selected mRNA was injected into Xenopus laevis oocytes, which subsequently secreted biologically active IL-1. The cDNA nucleotide sequence suggests that IL-1 is initially translated as a precursor molecule that is subsequently processed into the 15,000-20,000 Mr protein usually associated with IL-1 activity.

Bibliography

Auron, P. E., Webb, A. C., Rosenwasser, L. J., Mucci, S. F., Rich, A., Wolff, S. M., & Dinarello, C. A. (1984). Nucleotide sequence of human monocyte interleukin 1 precursor cDNA. Proceedings of the National Academy of Sciences, 81(24), 7907â7911.

Authors 7

P E Auron (first)
A C Webb (additional)
L J Rosenwasser (additional)
S F Mucci (additional)
A Rich (additional)
S M Wolff (additional)
C A Dinarello (additional)

References 0 Referenced 652

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 5:34 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:58 a.m.)
Indexed	1 week, 2 days ago (Aug. 21, 2025, 1:47 p.m.)
Issued	40 years, 8 months ago (Dec. 1, 1984)
Published	40 years, 8 months ago (Dec. 1, 1984)
Published Online	40 years, 8 months ago (Dec. 1, 1984)
Published Print	40 years, 8 months ago (Dec. 1, 1984)

Funders 0

None

BibTeX

@article{Auron_1984, title={Nucleotide sequence of human monocyte interleukin 1 precursor cDNA.}, volume={81}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.81.24.7907}, DOI={10.1073/pnas.81.24.7907}, number={24}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Auron, P E and Webb, A C and Rosenwasser, L J and Mucci, S F and Rich, A and Wolff, S M and Dinarello, C A}, year={1984}, month=dec, pages={7907–7911} }

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