Abstract
By using a reconstituted cell-free system we have demonstrated the existence of a soluble activity that is required for the export of proteins in Escherichia coli. This export factor sediments at about 12 S. It has been partially purified by passage through an omega-NH2-butylagarose column and by salt elution off a DEAE matrix. The export factor does not contain 6S RNA.
Dates
Type | When |
---|---|
Created | 19 years, 2 months ago (May 31, 2006, 5:34 a.m.) |
Deposited | 3 years, 4 months ago (April 13, 2022, 11:57 a.m.) |
Indexed | 1 year, 1 month ago (July 10, 2024, 4:33 p.m.) |
Issued | 40 years, 8 months ago (Dec. 1, 1984) |
Published | 40 years, 8 months ago (Dec. 1, 1984) |
Published Online | 40 years, 8 months ago (Dec. 1, 1984) |
Published Print | 40 years, 8 months ago (Dec. 1, 1984) |
@article{M_ller_1984, title={Protein export in Escherichia coli requires a soluble activity.}, volume={81}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.81.24.7737}, DOI={10.1073/pnas.81.24.7737}, number={24}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Müller, M and Blobel, G}, year={1984}, month=dec, pages={7737–7741} }