Abstract
The promoter-proximal portion of the operon containing the Escherichia coli nusA gene has been cloned. Its nucleotide sequence shows that genes for tRNAMetf2 and a 15-kilodalton protein of unknown function precede the nusA protein gene. The sequence suggests that the three genes form a single transcription unit. Consistent with this hypothesis, purified RNA polymerase formed full-length transcripts on the cloned DNA in vitro, although transcription was frequently arrested at the intercistronic site(s) between the gene for tRNAMetf2 and the 15-kilodalton protein.
Dates
Type | When |
---|---|
Created | 19 years, 3 months ago (May 31, 2006, 5:31 a.m.) |
Deposited | 3 years, 4 months ago (April 13, 2022, 11:59 a.m.) |
Indexed | 1 year, 6 months ago (Feb. 23, 2024, 1:31 p.m.) |
Issued | 41 years, 8 months ago (Jan. 1, 1984) |
Published | 41 years, 8 months ago (Jan. 1, 1984) |
Published Online | 41 years, 8 months ago (Jan. 1, 1984) |
Published Print | 41 years, 8 months ago (Jan. 1, 1984) |
@article{Ishii_1984, title={tRNAMetf2 gene in the leader region of the nusA operon in Escherichia coli.}, volume={81}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.81.2.409}, DOI={10.1073/pnas.81.2.409}, number={2}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Ishii, S and Kuroki, K and Imamoto, F}, year={1984}, month=jan, pages={409–413} }