DOI: 10.1073/pnas.81.2.357. Purification and characterization of acidic fibroblast growth factor from bovine brain.

Purification and characterization of acidic fibroblast growth factor from bovine brain.

10.1073/pnas.81.2.357

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Acidic brain fibroblast growth factor has been purified a minimum of 35,000-fold to apparent homogeneity by a combination of differential salt precipitation, ion exchange chromatography, gel filtration, isoelectric focusing, and hydrophobic chromatography on a C4 reversed-phase HPLC column. Two microheterogeneous forms of the molecule are obtained with apparent molecular masses of 16,600 and 16,800 daltons. The mitogen is highly active with half-maximal stimulation of BALB/c 3T3 fibroblasts at about 40 pg/ml in an assay using incorporation of [methyl-3H]thymidine into DNA.

Bibliography

Thomas, K. A., Rios-Candelore, M., & Fitzpatrick, S. (1984). Purification and characterization of acidic fibroblast growth factor from bovine brain. Proceedings of the National Academy of Sciences, 81(2), 357â361.

Authors 3

K A Thomas (first)
M Rios-Candelore (additional)
S Fitzpatrick (additional)

References 0 Referenced 167

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 5:31 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:58 a.m.)
Indexed	1 month, 1 week ago (July 20, 2025, 12:16 a.m.)
Issued	41 years, 8 months ago (Jan. 1, 1984)
Published	41 years, 8 months ago (Jan. 1, 1984)
Published Online	41 years, 8 months ago (Jan. 1, 1984)
Published Print	41 years, 8 months ago (Jan. 1, 1984)

Funders 0

None

BibTeX

@article{Thomas_1984, title={Purification and characterization of acidic fibroblast growth factor from bovine brain.}, volume={81}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.81.2.357}, DOI={10.1073/pnas.81.2.357}, number={2}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Thomas, K A and Rios-Candelore, M and Fitzpatrick, S}, year={1984}, month=jan, pages={357–361} }

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