DOI: 10.1073/pnas.81.17.5369. Expression of a biologically active fragment of human IgE epsilon chain in Escherichia coli.

Expression of a biologically active fragment of human IgE epsilon chain in Escherichia coli.

10.1073/pnas.81.17.5369

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

cDNA corresponding to human IgE heavy (epsilon) chain mRNA was cloned from human IgE-secreting myeloma U266 cells. Partial nucleotide sequence analysis demonstrated that the cloned cDNA contained the coding region for about two-thirds of the CH2 and all of the CH3 and CH4 domains as well as the 3'-untranslated region. This epsilon cDNA was inserted into expression vector pUC7 and expression of an epsilon-chain fragment in Escherichia coli was demonstrated by protein blot analysis using 125I-labeled goat anti-human IgE as probe. The expression product was purified on a column of goat anti-human IgE-conjugated Sepharose 4B and the polypeptide was found to retain binding activity to human basophils.

Bibliography

Liu, F. T., Albrandt, K. A., Bry, C. G., & Ishizaka, T. (1984). Expression of a biologically active fragment of human IgE epsilon chain in Escherichia coli. Proceedings of the National Academy of Sciences, 81(17), 5369â5373.

Authors 4

F T Liu (first)
K A Albrandt (additional)
C G Bry (additional)
T Ishizaka (additional)

References 0 Referenced 25

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 5:28 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:59 a.m.)
Indexed	4 months, 3 weeks ago (April 8, 2025, 6:05 p.m.)
Issued	41 years ago (Sept. 1, 1984)
Published	41 years ago (Sept. 1, 1984)
Published Online	41 years ago (Sept. 1, 1984)
Published Print	41 years ago (Sept. 1, 1984)

Funders 0

None

BibTeX

@article{Liu_1984, title={Expression of a biologically active fragment of human IgE epsilon chain in Escherichia coli.}, volume={81}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.81.17.5369}, DOI={10.1073/pnas.81.17.5369}, number={17}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Liu, F T and Albrandt, K A and Bry, C G and Ishizaka, T}, year={1984}, month=sep, pages={5369–5373} }

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