DOI: 10.1073/pnas.81.11.3273. Generation of antibody activity from immunoglobulin polypeptide chains produced in Escherichia coli.

Generation of antibody activity from immunoglobulin polypeptide chains produced in Escherichia coli.

10.1073/pnas.81.11.3273

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Plasmids have been constructed that direct the synthesis in Escherichia coli of heavy chains and/or light chains of an anti-carcinoembryonic antigen (CEA) antibody. Another plasmid was constructed for expression of a truncated form of heavy chain (Fd' fragment) in E. coli. Functional CEA-binding activity was obtained by in vitro reconstitution in E. coli extracts of heavy chain or Fd' fragment mixed with extracts containing light chain.

Bibliography

Cabilly, S., Riggs, A. D., Pande, H., Shively, J. E., Holmes, W. E., Rey, M., Perry, L. J., Wetzel, R., & Heyneker, H. L. (1984). Generation of antibody activity from immunoglobulin polypeptide chains produced in Escherichia coli. Proceedings of the National Academy of Sciences, 81(11), 3273â3277.

Authors 9

S Cabilly (first)
A D Riggs (additional)
H Pande (additional)
J E Shively (additional)
W E Holmes (additional)
M Rey (additional)
L J Perry (additional)
R Wetzel (additional)
H L Heyneker (additional)

References 0 Referenced 117

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 5:24 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:58 a.m.)
Indexed	4 weeks, 1 day ago (Aug. 2, 2025, 12:23 a.m.)
Issued	41 years, 2 months ago (June 1, 1984)
Published	41 years, 2 months ago (June 1, 1984)
Published Online	41 years, 2 months ago (June 1, 1984)
Published Print	41 years, 2 months ago (June 1, 1984)

Funders 0

None

BibTeX

@article{Cabilly_1984, title={Generation of antibody activity from immunoglobulin polypeptide chains produced in Escherichia coli.}, volume={81}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.81.11.3273}, DOI={10.1073/pnas.81.11.3273}, number={11}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Cabilly, S and Riggs, A D and Pande, H and Shively, J E and Holmes, W E and Rey, M and Perry, L J and Wetzel, R and Heyneker, H L}, year={1984}, month=jun, pages={3273–3277} }

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