DOI: 10.1073/pnas.80.9.2591. UV-induction of chalcone synthase mRNA in cell suspension cultures of <i>Petroselinum hortense</i>

UV-induction of chalcone synthase mRNA in cell suspension cultures of Petroselinum hortense

10.1073/pnas.80.9.2591

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

DNAs complementary to poly(A) + mRNAs from UV-irradiated cell suspension cultures of parsley ( Petroselinum hortense ) were inserted into pBR322 and used to transform Escherichia coli strain RR1. A clone containing a DNA complementary to chalcone synthase mRNA was identified by hybrid-selected and hybrid-arrested translation. Large and rapid changes in the amount of chalcone synthase mRNA in response to irradiation of the cells was detected by RNA blot hybridization experiments. The pattern of changes coincided with that previously determined for the rate of chalcone synthase synthesis as measured either in vivo or with polyribosomal mRNA in vitro . The results are consistent with the hypothesis that induction of chalcone synthase by UV light is due to a transient increase in the rate of synthesis of chalcone synthase mRNA.

Bibliography

Kreuzaler, F., Ragg, H., Fautz, E., Kuhn, D. N., & Hahlbrock, K. (1983). UV-induction of chalcone synthase mRNA in cell suspension cultures of Petroselinum hortense. Proceedings of the National Academy of Sciences, 80(9), 2591â2593.

Authors 5

Fritz Kreuzaler (first)
Hermann Ragg (additional)
Erich Fautz (additional)
David N. Kuhn (additional)
Klaus Hahlbrock (additional)

References 0 Referenced 117

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 5:21 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:03 p.m.)
Indexed	4 weeks ago (Aug. 5, 2025, 8:29 a.m.)
Issued	42 years, 4 months ago (May 1, 1983)
Published	42 years, 4 months ago (May 1, 1983)
Published Online	42 years, 4 months ago (May 1, 1983)
Published Print	42 years, 4 months ago (May 1, 1983)

Funders 0

None

BibTeX

@article{Kreuzaler_1983, title={UV-induction of chalcone synthase mRNA in cell suspension cultures of Petroselinum hortense}, volume={80}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.80.9.2591}, DOI={10.1073/pnas.80.9.2591}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Kreuzaler, Fritz and Ragg, Hermann and Fautz, Erich and Kuhn, David N. and Hahlbrock, Klaus}, year={1983}, month=may, pages={2591–2593} }

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