DOI: 10.1073/pnas.80.3.697. Gene for staphylococcal protein A.

10.1073/pnas.80.3.697

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The gene for protein A from Staphylococcus aureus was cloned into pBR322 in Escherichia coli. An immunoassay was used to detect production of the protein. Protein A produced in E. coli was found in the periplasmic space and was purified and concentrated by IgG-Sepharose affinity chromatography. DNA sequence assay of the gene revealed a region with the general features of a prokaryotic signal peptide and a fifth structural region homologous to the four repetitive regions found earlier by amino acid sequence determination of the mature protein. Upstream from the structural gene there is a possible promoter region and a ribosomal binding sequence typical of gram-positive bacteria. The initiation codon is TTG.

Bibliography

LÃ¶fdahl, S., Guss, B., UhlÃ©n, M., Philipson, L., & Lindberg, M. (1983). Gene for staphylococcal protein A. Proceedings of the National Academy of Sciences, 80(3), 697â701.

Authors 5

S Löfdahl (first)
B Guss (additional)
M Uhlén (additional)
L Philipson (additional)
M Lindberg (additional)

References 0 Referenced 153

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 3:24 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:43 a.m.)
Indexed	11 months, 3 weeks ago (Sept. 9, 2024, 10:46 a.m.)
Issued	42 years, 7 months ago (Feb. 1, 1983)
Published	42 years, 7 months ago (Feb. 1, 1983)
Published Online	42 years, 7 months ago (Feb. 1, 1983)
Published Print	42 years, 7 months ago (Feb. 1, 1983)

Funders 0

None

BibTeX

@article{L_fdahl_1983, title={Gene for staphylococcal protein A.}, volume={80}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.80.3.697}, DOI={10.1073/pnas.80.3.697}, number={3}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Löfdahl, S and Guss, B and Uhlén, M and Philipson, L and Lindberg, M}, year={1983}, month=feb, pages={697–701} }

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