DOI: 10.1073/pnas.80.2.477. Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyl transferase.

Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyl transferase.

10.1073/pnas.80.2.477

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We have cloned a full-length 1.6-kilobase cDNA of a human mRNA coding for hypoxanthine phosphoribosyltransferase (HPRT; IMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.8) into a simian virus 40-based expression vector and have determined its full nucleotide sequence. The inferred amino acid sequence agrees with a partial amino acid sequence determined for authentic human HPRT protein. Transfection of HPRT-deficient mouse LA9 cells with the purified plasmid leads to the expression of human HPRT enzyme activity in cells stably transfected and selected for enzyme activity in hypoxanthine/aminopterin/thymidine medium.

Bibliography

Jolly, D. J., Okayama, H., Berg, P., Esty, A. C., Filpula, D., Bohlen, P., Johnson, G. G., Shively, J. E., Hunkapillar, T., & Friedmann, T. (1983). Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyl transferase. Proceedings of the National Academy of Sciences, 80(2), 477â481.

Authors 10

D J Jolly (first)
H Okayama (additional)
P Berg (additional)
A C Esty (additional)
D Filpula (additional)
P Bohlen (additional)
G G Johnson (additional)
J E Shively (additional)
T Hunkapillar (additional)
T Friedmann (additional)

References 0 Referenced 186

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 5:14 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:47 a.m.)
Indexed	2 months, 3 weeks ago (June 3, 2025, 3:46 p.m.)
Issued	42 years, 7 months ago (Jan. 1, 1983)
Published	42 years, 7 months ago (Jan. 1, 1983)
Published Online	42 years, 7 months ago (Jan. 1, 1983)
Published Print	42 years, 7 months ago (Jan. 1, 1983)

Funders 0

None

BibTeX

@article{Jolly_1983, title={Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyl transferase.}, volume={80}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.80.2.477}, DOI={10.1073/pnas.80.2.477}, number={2}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Jolly, D J and Okayama, H and Berg, P and Esty, A C and Filpula, D and Bohlen, P and Johnson, G G and Shively, J E and Hunkapillar, T and Friedmann, T}, year={1983}, month=jan, pages={477–481} }

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