DOI: 10.1073/pnas.80.18.5680. Isolation and characterization of the RAD3 gene of Saccharomyces cerevisiae and inviability of rad3 deletion mutants

Isolation and characterization of the RAD3 gene of Saccharomyces cerevisiae and inviability of rad3 deletion mutants

10.1073/pnas.80.18.5680

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The RAD3 gene of Saccharomyces cerevisiae is required for nicking of DNA containing pyrimidine dimers or interstrand crosslinks. We have cloned the RAD3 gene and physically mapped it to 2.6 kilobase of DNA. A DNA segment of the cloned RAD3 insert was ligated into plasmid YIp5, which transforms yeast by homologous integration, and shown to integrate at the RAD3 site in chromosome V, thus verifying the cloned DNA segment to be the RAD3 gene and not a suppressor. The RAD3 gene encodes a 2.5-kilobase mRNA, extending between the Kpn I site and the Sau 3A1/ Bam HI fusion junction in plasmid pSP10, and the direction of transcription has been determined. The 2.5-kilobase transcript could encode a protein of about 90,000 daltons. We also show the deletions of the RAD3 gene to be recessive lethals, indicating that the RAD3 gene plays an important role in other cellular processes in addition to incision of damaged DNA.

Bibliography

Higgins, D. R., Prakash, S., Reynolds, P., Polakowska, R., Weber, S., & Prakash, L. (1983). Isolation and characterization of the RAD3 gene of Saccharomyces cerevisiae and inviability of rad3 deletion mutants. Proceedings of the National Academy of Sciences, 80(18), 5680â5684.

Authors 6

David R. Higgins (first)
Satya Prakash (additional)
Paul Reynolds (additional)
Renata Polakowska (additional)
Shane Weber (additional)
Louise Prakash (additional)

References 0 Referenced 65

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 5:13 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:42 a.m.)
Indexed	1 year, 10 months ago (Oct. 26, 2023, 9:57 p.m.)
Issued	41 years, 11 months ago (Sept. 1, 1983)
Published	41 years, 11 months ago (Sept. 1, 1983)
Published Online	41 years, 11 months ago (Sept. 1, 1983)
Published Print	41 years, 11 months ago (Sept. 1, 1983)

Funders 0

None

BibTeX

@article{Higgins_1983, title={Isolation and characterization of the RAD3 gene of Saccharomyces cerevisiae and inviability of rad3 deletion mutants}, volume={80}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.80.18.5680}, DOI={10.1073/pnas.80.18.5680}, number={18}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Higgins, David R. and Prakash, Satya and Reynolds, Paul and Polakowska, Renata and Weber, Shane and Prakash, Louise}, year={1983}, month=sep, pages={5680–5684} }

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