DOI: 10.1073/pnas.80.17.5190. Nucleotide sequence of a streptomycete aminoglycoside phosphotransferase gene and its relationship to phosphotransferases encoded by resistance plasmids.

Nucleotide sequence of a streptomycete aminoglycoside phosphotransferase gene and its relationship to phosphotransferases encoded by resistance plasmids.

10.1073/pnas.80.17.5190

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The DNA sequence of an aminoglycoside phosphotransferase gene (aph) from Streptomyces fradiae ATCC 10745 (a neomycin producer) was determined. The gene was localized by in vitro subcloning and insertional inactivation. Molecular weight, amino acid composition, and amino-terminal analysis of the purified aph gene product confirmed the accuracy and position of the aph gene sequence. Pairwise comparisons of S. fradiae aph with the aph genes encoded by bacterial transposons Tn5 and Tn903 showed significant nucleotide and amino acid homologies which indicated a common evolutionary origin for these antibiotic-resistance genes.

Bibliography

Thompson, C. J., & Gray, G. S. (1983). Nucleotide sequence of a streptomycete aminoglycoside phosphotransferase gene and its relationship to phosphotransferases encoded by resistance plasmids. Proceedings of the National Academy of Sciences, 80(17), 5190â5194.

Authors 2

C J Thompson (first)
G S Gray (additional)

References 0 Referenced 85

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 5:11 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:56 a.m.)
Indexed	1 month, 3 weeks ago (July 11, 2025, 6:17 a.m.)
Issued	42 years ago (Sept. 1, 1983)
Published	42 years ago (Sept. 1, 1983)
Published Online	42 years ago (Sept. 1, 1983)
Published Print	42 years ago (Sept. 1, 1983)

Funders 0

None

BibTeX

@article{Thompson_1983, title={Nucleotide sequence of a streptomycete aminoglycoside phosphotransferase gene and its relationship to phosphotransferases encoded by resistance plasmids.}, volume={80}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.80.17.5190}, DOI={10.1073/pnas.80.17.5190}, number={17}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Thompson, C J and Gray, G S}, year={1983}, month=sep, pages={5190–5194} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        11
      ]
    ],
    "date-time": "2025-07-11T10:17:52Z",
    "timestamp": 1752229072887
  },
  "reference-count": 0,
  "publisher": "Proceedings of the National Academy of Sciences",
  "issue": "17",
  "content-domain": {
    "domain": [
      "www.pnas.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1983,
        9
      ]
    ]
  },
  "abstract": "<jats:p>The DNA sequence of an aminoglycoside phosphotransferase gene (aph) from Streptomyces fradiae ATCC 10745 (a neomycin producer) was determined. The gene was localized by in vitro subcloning and insertional inactivation. Molecular weight, amino acid composition, and amino-terminal analysis of the purified aph gene product confirmed the accuracy and position of the aph gene sequence. Pairwise comparisons of S. fradiae aph with the aph genes encoded by bacterial transposons Tn5 and Tn903 showed significant nucleotide and amino acid homologies which indicated a common evolutionary origin for these antibiotic-resistance genes.</jats:p>",
  "DOI": "10.1073/pnas.80.17.5190",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2006,
        5,
        31
      ]
    ],
    "date-time": "2006-05-31T09:11:59Z",
    "timestamp": 1149066719000
  },
  "page": "5190-5194",
  "update-policy": "http://dx.doi.org/10.1073/pnas.cm10313",
  "source": "Crossref",
  "is-referenced-by-count": 85,
  "title": "Nucleotide sequence of a streptomycete aminoglycoside phosphotransferase gene and its relationship to phosphotransferases encoded by resistance plasmids.",
  "prefix": "10.1073",
  "volume": "80",
  "author": [
    {
      "given": "C J",
      "family": "Thompson",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "G S",
      "family": "Gray",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "341",
  "published-online": {
    "date-parts": [
      [
        1983,
        9
      ]
    ]
  },
  "container-title": "Proceedings of the National Academy of Sciences",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://pnas.org/doi/pdf/10.1073/pnas.80.17.5190",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2022,
        4,
        13
      ]
    ],
    "date-time": "2022-04-13T15:56:48Z",
    "timestamp": 1649865408000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://pnas.org/doi/full/10.1073/pnas.80.17.5190"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1983,
        9
      ]
    ]
  },
  "references-count": 0,
  "journal-issue": {
    "issue": "17",
    "published-print": {
      "date-parts": [
        [
          1983,
          9
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1073/pnas.80.17.5190"
  ],
  "URL": "http://dx.doi.org/10.1073/pnas.80.17.5190",
  "relation": {},
  "ISSN": [
    "0027-8424",
    "1091-6490"
  ],
  "subject": [],
  "container-title-short": "Proc. Natl. Acad. Sci. U.S.A.",
  "published": {
    "date-parts": [
      [
        1983,
        9
      ]
    ]
  },
  "assertion": [
    {
      "value": "1983-09-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}