Abstract
A naturally occurring opal suppressor serine tRNA has been purified from chicken liver and used as a probe to isolate the corresponding gene from a library of chicken DNA in bacteriophage lambda. This minor tRNA is encoded by a single-copy gene that is not part of a tRNA gene cluster. DNA sequence analysis of the gene and its flanking DNA segments shows that the gene is encoded in an 87-base-pair segment without intervening sequences and specifies a tRNA that reads the termination codon UGA. This gene has additional nucleotides in the 5' internal promoter region but has a normal 3' internal promoter sequence and the usual termination signal.
Dates
Type | When |
---|---|
Created | 19 years, 3 months ago (May 31, 2006, 5:11 a.m.) |
Deposited | 3 years, 4 months ago (April 13, 2022, 11:59 a.m.) |
Indexed | 1 month ago (Aug. 6, 2025, 8:01 a.m.) |
Issued | 42 years, 1 month ago (Aug. 1, 1983) |
Published | 42 years, 1 month ago (Aug. 1, 1983) |
Published Online | 42 years, 1 month ago (Aug. 1, 1983) |
Published Print | 42 years, 1 month ago (Aug. 1, 1983) |
@article{Hatfield_1983, title={Characterization and nucleotide sequence of a chicken gene encoding an opal suppressor tRNA and its flanking DNA segments.}, volume={80}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.80.16.4940}, DOI={10.1073/pnas.80.16.4940}, number={16}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Hatfield, D L and Dudock, B S and Eden, F C}, year={1983}, month=aug, pages={4940–4944} }