DOI: 10.1073/pnas.80.16.4922. 12-fold difference between the critical monomer concentrations of the two ends of actin filaments in physiological salt conditions.

12-fold difference between the critical monomer concentrations of the two ends of actin filaments in physiological salt conditions.

10.1073/pnas.80.16.4922

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We determined the critical monomer concentrations at which association and dissociation reactions are balanced at the two ends of actin filaments. For measurement of the critical concentration of the pointed end, interference with the high dynamics of the barbed end was excluded by capping the barbed ends with an actin filament capping protein isolated from bovine brain. The critical concentration of the pointed end (1.5 microM) was found to be 12- to 15-fold higher than the critical concentration of the barbed end (0.10-0.12 microM) at a temperature of 37 degrees C and physiological salt concentrations (100 mM KCl/1-2 mM MgCl2/0.3 mM EGTA or 0.2 mM CaCl2, pH 7.5).

Bibliography

Wegner, A., & Isenberg, G. (1983). 12-fold difference between the critical monomer concentrations of the two ends of actin filaments in physiological salt conditions. Proceedings of the National Academy of Sciences, 80(16), 4922â4925.

Authors 2

A Wegner (first)
G Isenberg (additional)

References 0 Referenced 68

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 5:11 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 12:01 p.m.)
Indexed	1 year ago (Aug. 9, 2024, 10:36 a.m.)
Issued	42 years ago (Aug. 1, 1983)
Published	42 years ago (Aug. 1, 1983)
Published Online	42 years ago (Aug. 1, 1983)
Published Print	42 years ago (Aug. 1, 1983)

Funders 0

None

BibTeX

@article{Wegner_1983, title={12-fold difference between the critical monomer concentrations of the two ends of actin filaments in physiological salt conditions.}, volume={80}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.80.16.4922}, DOI={10.1073/pnas.80.16.4922}, number={16}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Wegner, A and Isenberg, G}, year={1983}, month=aug, pages={4922–4925} }

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