DOI: 10.1073/pnas.79.3.754. In vitro reconstitution of intermediate filaments form mammalian neurofilament triplet polypeptides.

In vitro reconstitution of intermediate filaments form mammalian neurofilament triplet polypeptides.

10.1073/pnas.79.3.754

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Intermediate filaments (IF) were reconstituted in vitro from bovine neurofilament triplet polypeptides. Neural IF, solubilized in either low salt or 8 M urea solution, assembled into IF when returned to near-physiological solution conditions. The 68,000-dalton component of the triplet, purified to homogeneity by preparative NaDodSO4 electrophoresis, was renatured and reassembled into short (approximatley 0.05-micrometer) approximatley 10 nm-diameter filaments. These results demonstrate that the triplet polypeptides are components of neural IF and that the 68,000-dalton polypeptide is an IF structural protein.

Bibliography

Zackroff, R. V., Idler, W. W., Steinert, P. M., & Goldman, R. D. (1982). In vitro reconstitution of intermediate filaments form mammalian neurofilament triplet polypeptides. Proceedings of the National Academy of Sciences, 79(3), 754â757.

Authors 4

R V Zackroff (first)
W W Idler (additional)
P M Steinert (additional)
R D Goldman (additional)

References 0 Referenced 46

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 3:16 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:46 a.m.)
Indexed	1 year, 3 months ago (May 9, 2024, 12:37 a.m.)
Issued	43 years, 6 months ago (Feb. 1, 1982)
Published	43 years, 6 months ago (Feb. 1, 1982)
Published Online	43 years, 6 months ago (Feb. 1, 1982)
Published Print	43 years, 6 months ago (Feb. 1, 1982)

Funders 0

None

BibTeX

@article{Zackroff_1982, title={In vitro reconstitution of intermediate filaments form mammalian neurofilament triplet polypeptides.}, volume={79}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.79.3.754}, DOI={10.1073/pnas.79.3.754}, number={3}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Zackroff, R V and Idler, W W and Steinert, P M and Goldman, R D}, year={1982}, month=feb, pages={754–757} }

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