DOI: 10.1073/pnas.79.19.5896. DNA motions in the nucleosome core particle.

DNA motions in the nucleosome core particle.

10.1073/pnas.79.19.5896

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

We have used time-resolved triplet state anisotropy decay techniques to measure the conformational flexibility of DNA in the nucleosome. From these measurements we conclude that, in a nucleosome, the DNA helix experiences substantial internal flexibility, which occurs with a time constant near 30 nsec. We find that our data can be fit well by a modified version of the Barkley-Zimm model for DNA motion, allowing only DNA twisting motions and the overall tumbling of the nucleosome. That fit yields a calculated torsional rigidity equal to 1.8 X 10(-19) erg X cm, a value equal to that measured for uncomplexed DNA. We conclude from such similarity that large, fast twisting motions of the DNA helix persist, nearly unaltered, when DNA is wrapped to form a nucleosome.

Bibliography

Wang, J., Hogan, M., & Austin, R. H. (1982). DNA motions in the nucleosome core particle. Proceedings of the National Academy of Sciences, 79(19), 5896â5900.

Authors 3

J Wang (first)
M Hogan (additional)
R H Austin (additional)

References 0 Referenced 37

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 4:55 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:27 a.m.)
Indexed	1 year, 6 months ago (Feb. 9, 2024, 9:44 p.m.)
Issued	42 years, 11 months ago (Oct. 1, 1982)
Published	42 years, 11 months ago (Oct. 1, 1982)
Published Online	42 years, 11 months ago (Oct. 1, 1982)
Published Print	42 years, 11 months ago (Oct. 1, 1982)

Funders 0

None

BibTeX

@article{Wang_1982, title={DNA motions in the nucleosome core particle.}, volume={79}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.79.19.5896}, DOI={10.1073/pnas.79.19.5896}, number={19}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Wang, J and Hogan, M and Austin, R H}, year={1982}, month=oct, pages={5896–5900} }

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