DOI: 10.1073/pnas.78.9.5578. Mg <sup>2+</sup> /KCl-ATPase of plant plasma membranes is an electrogenic pump

Mg 2+ /KCl-ATPase of plant plasma membranes is an electrogenic pump

10.1073/pnas.78.9.5578

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The function of the Mg 2+ -requiring KCl-stimulated ATPase (ATP phosphohydrolase, EC 3.6.1.3) of higher plants in active ion transport was investigated by using a purified microsomal fraction containing sealed plasma membrane vesicles. (Sze, H. (1980) Proc. Natl. Acad. Sci. USA 77, 5904-5908). A transmembrane electrical potential (+30 to +44 mV), monitored by uptake of a permeant anion ( 35 SCN - ), was generated specifically by ATP in purified microsomal vesicles of tobacco callus. ATP-dependent 35 SCN - uptake required Mg 2+ , was optimal at pH 6.75, and showed similar ATP concentration dependence as the Mg 2+ -requiring KCl-stimulated ATPase activity. Plasma membrane ATPase inhibitors ( N,N ′-dicyclohexylcarbodiimide and vanadate) inhibited generation of the ATP-dependent electrical potential. A proton conductor (carbonyl cyanide m -chlorophenylhydrazone), but not a K + ionophore (valinomycin), completely collapsed the electrical potential. The results provide in vitro evidence that the Mg 2+ /KCl-ATPase of higher plants is an electrogenic pump. These results are consistent with the hypothesis that an electrogenic H + pump is catalyzed by the plasma membrane ATPase of plants.

Bibliography

Sze, H., & Churchill, K. A. (1981). Mg 2+ /KCl-ATPase of plant plasma membranes is an electrogenic pump. Proceedings of the National Academy of Sciences, 78(9), 5578â5582.

Authors 2

Heven Sze (first)
Kathleen A. Churchill (additional)

References 0 Referenced 43

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 4:49 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:33 a.m.)
Indexed	2 months, 2 weeks ago (June 10, 2025, 7:43 p.m.)
Issued	43 years, 11 months ago (Sept. 1, 1981)
Published	43 years, 11 months ago (Sept. 1, 1981)
Published Online	43 years, 11 months ago (Sept. 1, 1981)
Published Print	43 years, 11 months ago (Sept. 1, 1981)

Funders 0

None

BibTeX

@article{Sze_1981, title={Mg 2+ /KCl-ATPase of plant plasma membranes is an electrogenic pump}, volume={78}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.78.9.5578}, DOI={10.1073/pnas.78.9.5578}, number={9}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Sze, Heven and Churchill, Kathleen A.}, year={1981}, month=sep, pages={5578–5582} }

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