DOI: 10.1073/pnas.78.8.4777. 7-Methylguanine in poly(dG-dC).poly(dG-dC) facilitates z-DNA formation.

7-Methylguanine in poly(dG-dC).poly(dG-dC) facilitates z-DNA formation.

10.1073/pnas.78.8.4777

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Poly(dG-dC).poly(dG-dC) was methylated at the N-7 position of guanine through the use of dimethyl sulfate. The conversion of poly(dG-dC).poly(dG-dC) from B-DNA to the Z-DNA form was followed by measuring both the circular dichroic spectra and changes in the absorbance ratio A295/A260. Increasing methylation steadily decreases the amount of NaCl or MgCl2 that is required to convert the polymer from B-DNA to Z-DNA. At 100% methylation of the guanine residues, the modified polymer is fully converted to Z-DNA in a physiological salt solution. Kinetic experiments show that methylation markedly accelerates the speed of the conversion from B-DNA to Z-DNA in the presence of added salt. These effects may be partly due to the positive charge on guanine that accompanies N-7 methylation.

Bibliography

MÃ¶ller, A., Nordheim, A., Nichols, S. R., & Rich, A. (1981). 7-Methylguanine in poly(dG-dC).poly(dG-dC) facilitates z-DNA formation. Proceedings of the National Academy of Sciences, 78(8), 4777â4781.

Authors 4

A Möller (first)
A Nordheim (additional)
S R Nichols (additional)
A Rich (additional)

References 0 Referenced 54

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 4:47 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:31 a.m.)
Indexed	1 year, 9 months ago (Nov. 22, 2023, 10:59 p.m.)
Issued	44 years, 1 month ago (Aug. 1, 1981)
Published	44 years, 1 month ago (Aug. 1, 1981)
Published Online	44 years, 1 month ago (Aug. 1, 1981)
Published Print	44 years, 1 month ago (Aug. 1, 1981)

Funders 0

None

BibTeX

@article{M_ller_1981, title={7-Methylguanine in poly(dG-dC).poly(dG-dC) facilitates z-DNA formation.}, volume={78}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.78.8.4777}, DOI={10.1073/pnas.78.8.4777}, number={8}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Möller, A and Nordheim, A and Nichols, S R and Rich, A}, year={1981}, month=aug, pages={4777–4781} }

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