DOI: 10.1073/pnas.78.6.3469. Partial characterization of gamma (immune) interferon mRNA extracted from human lymphocytes.

Partial characterization of gamma (immune) interferon mRNA extracted from human lymphocytes.

10.1073/pnas.78.6.3469

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

gamma (immune) interferon (IFN-gamma) was induced in cultures of fresh human lymphocytes by combined treatment with a phorbol ester (12-O-tetradecanoylphorbol 13-acetate, TPA) and the T cell mitogen phytohemagglutinin (PHA). Compared to the IFN-gamma yields obtained with PHA induction alone, the inclusion of TPA caused a significant enhancement of IFN-gamma production. Poly(A)-containing mRNA was isolated from mononuclear cells induced with TPA and PHA. Injected into Xenopus laevis oocytes, this mRNA preparation gave rise to IFN activity with characteristic properties of human IFN-gamma. Sucrose density gradient centrifugation analysis showed that IFN-gamma mRNA sedimented at 15 S, suggesting that it contains a total of about 1400 nucleotides.

Bibliography

Taniguchi, T., Pang, R. H., Yip, Y. K., Henriksen, D., & Vilcek, J. (1981). Partial characterization of gamma (immune) interferon mRNA extracted from human lymphocytes. Proceedings of the National Academy of Sciences, 78(6), 3469â3472.

Authors 5

T Taniguchi (first)
R H Pang (additional)
Y K Yip (additional)
D Henriksen (additional)
J Vilcek (additional)

References 0 Referenced 28

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 4:43 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:23 a.m.)
Indexed	1 year, 9 months ago (Nov. 22, 2023, 4:03 p.m.)
Issued	44 years, 3 months ago (June 1, 1981)
Published	44 years, 3 months ago (June 1, 1981)
Published Online	44 years, 3 months ago (June 1, 1981)
Published Print	44 years, 3 months ago (June 1, 1981)

Funders 0

None

BibTeX

@article{Taniguchi_1981, title={Partial characterization of gamma (immune) interferon mRNA extracted from human lymphocytes.}, volume={78}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.78.6.3469}, DOI={10.1073/pnas.78.6.3469}, number={6}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Taniguchi, T and Pang, R H and Yip, Y K and Henriksen, D and Vilcek, J}, year={1981}, month=jun, pages={3469–3472} }

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