DOI: 10.1073/pnas.78.4.2164. Identification and localization of a gene that specifies production of Escherichia coli DNA topoisomerase I.

Identification and localization of a gene that specifies production of Escherichia coli DNA topoisomerase I.

10.1073/pnas.78.4.2164

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A gene that specifies production of Escherichia coli DNA topoisomerase I (omega protein) was identified with the aid of a radioimmunoassay for this protein. E. coli DNA topoisomerase I was produced by Salmonella typhimurium merodiploids that harbored E. coli plasmid F' 123, but not by strains that lost this plasmid. Analysis of strains with spontaneous deletions of F' 123 showed that the gene, topA, required for production of the E. coli omega protein was between the trp operon and the cysB gene. Deletions that eliminated topA also eliminated the supX gene. We suggest that topA is the structural gene of E. coli DNA topoisomerase I and that topA is identical to supX.

Bibliography

Trucksis, M., & Depew, R. E. (1981). Identification and localization of a gene that specifies production of Escherichia coli DNA topoisomerase I. Proceedings of the National Academy of Sciences, 78(4), 2164â2168.

Authors 2

M Trucksis (first)
R E Depew (additional)

References 0 Referenced 47

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 4:41 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:38 a.m.)
Indexed	1 year, 10 months ago (Oct. 27, 2023, 12:55 a.m.)
Issued	44 years, 5 months ago (April 1, 1981)
Published	44 years, 5 months ago (April 1, 1981)
Published Online	44 years, 5 months ago (April 1, 1981)
Published Print	44 years, 5 months ago (April 1, 1981)

Funders 0

None

BibTeX

@article{Trucksis_1981, title={Identification and localization of a gene that specifies production of Escherichia coli DNA topoisomerase I.}, volume={78}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.78.4.2164}, DOI={10.1073/pnas.78.4.2164}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Trucksis, M and Depew, R E}, year={1981}, month=apr, pages={2164–2168} }

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