DOI: 10.1073/pnas.78.4.2110. Amplification of the metallothionein-I gene in cadmium-resistant mouse cells.

Amplification of the metallothionein-I gene in cadmium-resistant mouse cells.

10.1073/pnas.78.4.2110

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Friend leukemia cells resistant to cadmium toxicity were selected. More than 70% of total cysteine incorporation in these cells was into the metal-binding protein, metallothionein. We used cDNA and genomic DNA clones containing the metallothionein-I gene to measure the concentration of its mRNA, the rate of gene transcription, and the number of genes. On a per cell basis, optimally induced, cadmium-resistant cells have a 14-fold more metallothionein-I mRNA, a 6-fold higher rate of metallothionein-I gene transcription, and 6-fold more metallothionein-I genes than do nonresistant cells. Metaphase spreads revealed that the resistant cells are nearly tetraploid and contain, on the average, three very small chromosomes that are absent from non-resistant Friend cells.

Bibliography

Beach, L. R., & Palmiter, R. D. (1981). Amplification of the metallothionein-I gene in cadmium-resistant mouse cells. Proceedings of the National Academy of Sciences, 78(4), 2110â2114.

Authors 2

L R Beach (first)
R D Palmiter (additional)

References 0 Referenced 203

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 4:41 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:40 a.m.)
Indexed	2 months ago (July 4, 2025, 7:48 a.m.)
Issued	44 years, 5 months ago (April 1, 1981)
Published	44 years, 5 months ago (April 1, 1981)
Published Online	44 years, 5 months ago (April 1, 1981)
Published Print	44 years, 5 months ago (April 1, 1981)

Funders 0

None

BibTeX

@article{Beach_1981, title={Amplification of the metallothionein-I gene in cadmium-resistant mouse cells.}, volume={78}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.78.4.2110}, DOI={10.1073/pnas.78.4.2110}, number={4}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Beach, L R and Palmiter, R D}, year={1981}, month=apr, pages={2110–2114} }

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