DOI: 10.1073/pnas.78.12.7727. High-resolution analysis of human peripheral lymphocyte chromosomes by flow cytometry.

High-resolution analysis of human peripheral lymphocyte chromosomes by flow cytometry.

10.1073/pnas.78.12.7727

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

A method for high-resolution analysis of the human karyotype by flow cytometry has been developed. Metaphase chromosomes are prepared from short-term peripheral blood cultures, stained with ethidium bromide, and analyzed on a standard fluorescence-activated cell sorter (FACS-II). Flow karyotypes with up to 20 peaks can be obtained with coefficients of variation in the range 1-2%. At this level of resolution the contribution of many of the human chromosomes can be evaluated separately. Significant and reproducible differences between normal individuals have been detected and have been correlated with differences in the centric heterochromatin of certain chromosomes as revealed in their C-banded karyotypes.

Bibliography

Young, B. D., Ferguson-Smith, M. A., Sillar, R., & Boyd, E. (1981). High-resolution analysis of human peripheral lymphocyte chromosomes by flow cytometry. Proceedings of the National Academy of Sciences, 78(12), 7727â7731.

Authors 4

B D Young (first)
M A Ferguson-Smith (additional)
R Sillar (additional)
E Boyd (additional)

References 0 Referenced 85

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 4:36 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:30 a.m.)
Indexed	1 month, 3 weeks ago (July 12, 2025, 6:58 p.m.)
Issued	43 years, 9 months ago (Dec. 1, 1981)
Published	43 years, 9 months ago (Dec. 1, 1981)
Published Online	43 years, 9 months ago (Dec. 1, 1981)
Published Print	43 years, 9 months ago (Dec. 1, 1981)

Funders 0

None

BibTeX

@article{Young_1981, title={High-resolution analysis of human peripheral lymphocyte chromosomes by flow cytometry.}, volume={78}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.78.12.7727}, DOI={10.1073/pnas.78.12.7727}, number={12}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Young, B D and Ferguson-Smith, M A and Sillar, R and Boyd, E}, year={1981}, month=dec, pages={7727–7731} }

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