DOI: 10.1073/pnas.78.12.7261. Yeast 2-micrometer plasmid DNA replication in vitro: origin and direction.

Yeast 2-micrometer plasmid DNA replication in vitro: origin and direction.

10.1073/pnas.78.12.7261

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

Most yeast strains harbor extrachromosomal 2-micrometer DNA, and this DNA synthesis, like nuclear DNA replication, is strictly under cell cycle control. A soluble extract of yeast Saccharomyces cerevisiae carries out semiconservative replication of added 2-micrometer DNA and Escherichia coli chimeric plasmids containing the 2-micrometer DNA. Replication is initiated on 10% of the DNA, and one round of replication is completed. The major products in early stages of replication are theta ("eye") forms which originate 140 +/- 50 nucleotides within one of the 599-base-pair inverted repeats of 2-micrometer DNA. Their replication is bidirectional and discontinuous. Extracts prepared from the cell division cycle mutant cdc8 show temperature-sensitive 2-micrometer DNA synthesis in vitro, suggesting that this in vitro system resembles in vivo 2-micrometer plasmid DNA replication. This system should provide a useful assay for the purification and characterization of yeast DNA replication proteins.

Bibliography

Kojo, H., Greenberg, B. D., & Sugino, A. (1981). Yeast 2-micrometer plasmid DNA replication in vitro: origin and direction. Proceedings of the National Academy of Sciences, 78(12), 7261â7265.

Authors 3

H Kojo (first)
B D Greenberg (additional)
A Sugino (additional)

References 0 Referenced 46

None

Dates

Type	When
Created	19 years, 2 months ago (May 31, 2006, 4:36 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:31 a.m.)
Indexed	1 year, 10 months ago (Oct. 22, 2023, 10:29 a.m.)
Issued	43 years, 8 months ago (Dec. 1, 1981)
Published	43 years, 8 months ago (Dec. 1, 1981)
Published Online	43 years, 8 months ago (Dec. 1, 1981)
Published Print	43 years, 8 months ago (Dec. 1, 1981)

Funders 0

None

BibTeX

@article{Kojo_1981, title={Yeast 2-micrometer plasmid DNA replication in vitro: origin and direction.}, volume={78}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.78.12.7261}, DOI={10.1073/pnas.78.12.7261}, number={12}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Kojo, H and Greenberg, B D and Sugino, A}, year={1981}, month=dec, pages={7261–7265} }

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