DOI: 10.1073/pnas.78.1.459. Excision of transposon Tn5 is dependent on the inverted repeats but not on the transposase function of Tn5.

Excision of transposon Tn5 is dependent on the inverted repeats but not on the transposase function of Tn5.

10.1073/pnas.78.1.459

Crossref journal-article

Proceedings of the National Academy of Sciences

Proceedings of the National Academy of Sciences (341)

Abstract

The excision of Tn5 from sites of insertion in the Escherichia coli genome was studied by examining the reversion of lac::Tn5 insertion mutations to lac+. We find that: (i) the frequency of excision depends on the site of Tn5 insertion, (ii) excision occurs efficiently in recA- cells, (iii) excision does not require a Tn5-encoded transposition function, and (iv) efficient excision requires the inverted repeats of Tn5. We propose that excision of Tn5 is similar to the formation of spontaneous deletions and occurs by slippage during DNA synthesis.

Bibliography

Egner, C., & Berg, D. E. (1981). Excision of transposon Tn5 is dependent on the inverted repeats but not on the transposase function of Tn5. Proceedings of the National Academy of Sciences, 78(1), 459â463.

Authors 2

C Egner (first)
D E Berg (additional)

References 0 Referenced 66

None

Dates

Type	When
Created	19 years, 3 months ago (May 31, 2006, 4:27 a.m.)
Deposited	3 years, 4 months ago (April 13, 2022, 11:34 a.m.)
Indexed	1 year, 2 months ago (June 3, 2024, 12:30 a.m.)
Issued	44 years, 8 months ago (Jan. 1, 1981)
Published	44 years, 8 months ago (Jan. 1, 1981)
Published Online	44 years, 8 months ago (Jan. 1, 1981)
Published Print	44 years, 8 months ago (Jan. 1, 1981)

Funders 0

None

BibTeX

@article{Egner_1981, title={Excision of transposon Tn5 is dependent on the inverted repeats but not on the transposase function of Tn5.}, volume={78}, ISSN={1091-6490}, url={http://dx.doi.org/10.1073/pnas.78.1.459}, DOI={10.1073/pnas.78.1.459}, number={1}, journal={Proceedings of the National Academy of Sciences}, publisher={Proceedings of the National Academy of Sciences}, author={Egner, C and Berg, D E}, year={1981}, month=jan, pages={459–463} }

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